Cytochrome P450 4F8 (EC 1.14.14.1) (CYPIVF8)
1_MSLLS 6_ LSWLG 11_ LRPVA 16_ ASPWL 21_ LLLVV 26_ GASWL 31_ LARIL 36_ AWTYA 41_ FYHNG 46_ RRLRC 51_ FPQPR 56_ KQNWF 61_ LGHLG 66_ LVTPT 71_ EEGLR 76_ VLTQL 81_ VATYP 86_ QGFVR 91_ WLGPI 96_ TPIIN 101_ LCHPD 106_ IVRSV 111_ INTSD 116_ AITDK 121_ DIVFY 126_ KTLKP 131_ WLGDG 136_ LLLSV 141_ GDKWR 146_ HHRRL 151_ LTPAF 156_ HFNIL 161_ KPYIK 166_ IFSKS 171_ ANIMH 176_ AKWQR 181_ LAMEG 186_ STCLD 191_ VFEHI 196_ SLMTL 201_ DSLQK 206_ CIFSF 211_ DSNCQ 216_ EKPSE 221_ YITAI 226_ MELSA 231_ LVVKR 236_ NNQFF 241_ RYKDF 246_ LYFLT 251_ PCGRR 256_ FHRAC 261_ RLVHD 266_ FTDAV 271_ IQERR 276_ RTLTS 281_ QGVDD 286_ FLQAK 291_ AKSKT 296_ LDFID 301_ VLLLS 306_ EDKNG 311_ KELSD 316_ EDIRA 321_ EADTF 326_ MFGGH 331_ DTTAS 336_ GLSWV 341_ LYNLA 346_ RHPEY 351_ QERCR 356_ QEVQE 361_ LLKDR 366_ EPKEI 371_ EWDDL 376_ AQLPF 381_ LTMCL 386_ KESLR 391_ LHPPI 396_ PTFAR 401_ GCTQD 406_ VVLPD 411_ SRVIP 416_ KGNVC 421_ NINIF 426_ AIHHN 431_ PSVWP 436_ DPEVY 441_ DPFRF 446_ DPENA 451_ QKRSP 456_ MAFIP 461_ FSAGP 466_ RNCIG 471_ QKFAM 476_ AEMKV 481_ VLALT 486_ LLRFR 491_ ILPDH 496_ REPRR 501_ TPEIV 506_ LRAED 511_GLWLR
1: A cytochrome P450 monooxygenase involved in the metabolism of endogenous polyunsaturated fatty acids (PUFAs) and their oxygenated derivatives (oxylipins). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Catalyzes the hydroxylation of carbon hydrogen bonds, with preference for omega-1 and omega-2 positions (PubMed:10791960, PubMed:15789615, PubMed:16112640). Hydroxylates (5Z,8Z,11Z,14Z)-eicosatetraenoic acid (arachidonate) predominantly at omega-2 position to form (18R)-hydroxyeicosatetraenoic acid (18R-HETE) (PubMed:10791960). Exhibits omega-1 hydroxylase activity toward prostaglandin (PG) H1, PGH2 and PGI2 (PubMed:10791960, PubMed:15789615). Catalyzes the epoxidation of double bonds of PUFAs, including docosahexaenoic and docosapentaenoic acids (PubMed:16112640). Shows little activity against PGD2, PGE1, PGE2, PGF2alpha, and leukotriene B4