Transcription initiation factor IIB (EC 2.3.1.48) (General transcription factor TFIIB) (S300-II)
1_MASTS 6_ RLDAL 11_ PRVTC 16_ PNHPD 21_ AILVE 26_ DYRAG 31_ DMICP 36_ ECGLV 41_ VGDRV 46_ IDVGS 51_ EWRTF 56_ SNDKA 61_ TKDPS 66_ RVGDS 71_ QNPLL 76_ SDGDL 81_ STMIG 86_ KGTGA 91_ ASFDE 96_ FGNSK 101_ YQNRR 106_ TMSSS 111_ DRAMM 116_ NAFKE 121_ ITTMA 126_ DRINL 131_ PRNIV 136_ DRTNN 141_ LFKQV 146_ YEQKS 151_ LKGRA 156_ NDAIA 161_ SACLY 166_ IACRQ 171_ EGVPR 176_ TFKEI 181_ CAVSR 186_ ISKKE 191_ IGRCF 196_ KLILK 201_ ALETS 206_ VDLIT 211_ TGDFM 216_ SRFCS 221_ NLCLP 226_ KQVQM 231_ AATHI 236_ ARKAV 241_ ELDLV 246_ PGRSP 251_ ISVAA 256_ AAIYM 261_ ASQAS 266_ AEKRT 271_ QKEIG 276_ DIAGV 281_ ADVTI 286_ RQSYR 291_ LIYPR 296_ APDLF 301_ PTDFK 306_ FDTPV 311_DKLPQ
1: General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA (PubMed:12931194, PubMed:1517211, PubMed:1876184, PubMed:1946368, PubMed:27193682, PubMed:3029109, PubMed:3818643, PubMed:7601352, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:9420329). Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed:8413225, PubMed:8504927, PubMed:8515820, PubMed:8516311, PubMed:8516312). Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed:7601352). Associates with chromatin to core promoter-specific regions (PubMed:12931194, PubMed:24441171). Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element (PubMed:10619841, PubMed:16230532, PubMed:7675079, PubMed:9420329). Modulates transcription start site selection (PubMed:10318856). Also exhibits autoacetyltransferase activity that contributes to the activated transcription (PubMed:12931194)