Serine/arginine-rich splicing factor 2 (Protein PR264) (Splicing component, 35 kDa) (Splicing factor SC35) (SC-35) (Splicing factor, arginine/serine-rich 2)
1_MSYGR 6_ PPPDV 11_ EGMTS 16_ LKVDN 21_ LTYRT 26_ SPDTL 31_ RRVFE 36_ KYGRV 41_ GDVYI 46_ PRDRY 51_ TKESR 56_ GFAFV 61_ RFHDK 66_ RDAED 71_ AMDAM 76_ DGAVL 81_ DGREL 86_ RVQMA 91_ RYGRP 96_ PDSHH 101_ SRRGP 106_ PPRRY 111_ GGGGY 116_ GRRSR 121_ SPRRR 126_ RRSRS 131_ RSRSR 136_ SRSRS 141_ RSRYS 146_ RSKSR 151_ SRTRS 156_ RSRST 161_ SKSRS 166_ ARRSK 171_ SKSSS 176_ VSRSR 181_ SRSRS 186_ RSRSR 191_ SPPPV 196_ SKRES 201_ KSRSR 206_ SKSPP 211_ KSPEE 216_EGAVS
1: Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment