Runt-related transcription factor 1 (Acute myeloid leukemia 1 protein) (Core-binding factor subunit alpha-2) (CBF-alpha-2) (Oncogene AML-1) (Polyomavirus enhancer-binding protein 2 alpha B subunit) (PEA2-alpha B) (PEBP2-alpha B) (SL3-3 enhancer factor 1 alpha B subunit) (SL3/AKV core-binding factor alpha B subunit)
1_MRIPV 6_ DASTS 11_ RRFTP 16_ PSTAL 21_ SPGKM 26_ SEALP 31_ LGAPD 36_ AGAAL 41_ AGKLR 46_ SGDRS 51_ MVEVL 56_ ADHPG 61_ ELVRT 66_ DSPNF 71_ LCSVL 76_ PTHWR 81_ CNKTL 86_ PIAFK 91_ VVALG 96_ DVPDG 101_ TLVTV 106_ MAGND 111_ ENYSA 116_ ELRNA 121_ TAAMK 126_ NQVAR 131_ FNDLR 136_ FVGRS 141_ GRGKS 146_ FTLTI 151_ TVFTN 156_ PPQVA 161_ TYHRA 166_ IKITV 171_ DGPRE 176_ PRRHR 181_ QKLDD 186_ QTKPG 191_ SLSFS 196_ ERLSE 201_ LEQLR 206_ RTAMR 211_ VSPHH 216_ PAPTP 221_ NPRAS 226_ LNHST 231_ AFNPQ 236_ PQSQM 241_ QDTRQ 246_ IQPSP 251_ PWSYD 256_ QSYQY 261_ LGSIA 266_ SPSVH 271_ PATPI 276_ SPGRA 281_ SGMTT 286_ LSAEL 291_ SSRLS 296_ TAPDL 301_ TAFSD 306_ PRQFP 311_ ALPSI 316_ SDPRM 321_ HYPGA 326_ FTYSP 331_ TPVTS 336_ GIGIG 341_ MSAMG 346_ SATRY 351_ HTYLP 356_ PPYPG 361_ SSQAQ 366_ GGPFQ 371_ ASSPS 376_ YHLYY 381_ GASAG 386_ SYQFS 391_ MVGGE 396_ RSPPR 401_ ILPPC 406_ TNAST 411_ GSALL 416_ NPSLP 421_ NQSDV 426_ VEAEG 431_ SHSNS 436_ PTNMA 441_ PSARL 446_EEAVW
1: Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (Probable). Essential for the development of normal hematopoiesis (PubMed:17431401). Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the BLK promoter (PubMed:10207087, PubMed:14970218). Inhibits KAT6B-dependent transcriptional activation (By similarity). Involved in lineage commitment of immature T cell precursors. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing (By similarity). Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down-regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells (PubMed:17377532). Positively regulates the expression of RORC in T-helper 17 cells (By similarity)
2: Isoform AML-1G shows higher binding activities for target genes and binds TCR-beta-E2 and RAG-1 target site with threefold higher affinity than other isoforms. It is less effective in the context of neutrophil terminal differentiation
3: Isoform AML-1L interferes with the transactivation activity of RUNX1