Single-stranded DNA-binding protein, mitochondrial (Mt-SSB) (MtSSB) (PWP1-interacting protein 17)
1_MFRRP 6_ VLQVL 11_ RQFVR 16_ HESET 21_ TTSLV 26_ LERSL 31_ NRVHL 36_ LGRVG 41_ QDPVL 46_ RQVEG 51_ KNPVT 56_ IFSLA 61_ TNEMW 66_ RSGDS 71_ EVYQL 76_ GDVSQ 81_ KTTWH 86_ RISVF 91_ RPGLR 96_ DVAYQ 101_ YVKKG 106_ SRIYL 111_ EGKID 116_ YGEYM 121_ DKNNV 126_ RRQAT 131_ TIIAD 136_ NIIFL 141_SDQTK
1: Binds preferentially and cooperatively to pyrimidine rich single-stranded DNA (ss-DNA) (PubMed:21953457, PubMed:23290262, PubMed:31550240). In vitro, required to maintain the copy number of mitochondrial DNA (mtDNA) and plays a crucial role during mtDNA replication by stimulating the activity of the replisome components POLG and TWNK at the replication fork (PubMed:12975372, PubMed:15167897, PubMed:21953457, PubMed:26446790, PubMed:31550240). Promotes the activity of the gamma complex polymerase POLG, largely by organizing the template DNA and eliminating secondary structures to favor ss-DNA conformations that facilitate POLG activity (PubMed:21953457, PubMed:26446790, PubMed:31550240). In addition it is able to promote the 5'-3' unwinding activity of the mtDNA helicase TWNK (PubMed:12975372). May also function in mtDNA repair (PubMed:23290262)