Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase (EC 2.4.1.144) (N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III) (GNT-III) (GlcNAc-T III) (N-acetylglucosaminyltransferase III)
1_MKMRR 6_ YKLFL 11_ MFCMA 16_ GLCLI 21_ SFLHF 26_ FKTLS 31_ YVTFP 36_ RELAS 41_ LSPNL 46_ VSSFF 51_ WNNAP 56_ VTPQA 61_ SPEPG 66_ GPDLL 71_ RTPLY 76_ SHSPL 81_ LQPLP 86_ PSKAA 91_ EELHR 96_ VDLVL 101_ PEDTT 106_ EYFVR 111_ TKAGG 116_ VCFKP 121_ GTKML 126_ ERPPP 131_ GRPEE 136_ KPEGA 141_ NGSSA 146_ RRPPR 151_ YLLSA 156_ RERTG 161_ GRGAR 166_ RKWVE 171_ CVCLP 176_ GWHGP 181_ SCGVP 186_ TVVQY 191_ SNLPT 196_ KERLV 201_ PREVP 206_ RRVIN 211_ AINVN 216_ HEFDL 221_ LDVRF 226_ HELGD 231_ VVDAF 236_ VVCES 241_ NFTAY 246_ GEPRP 251_ LKFRE 256_ MLTNG 261_ TFEYI 266_ RHKVL 271_ YVFLD 276_ HFPPG 281_ GRQDG 286_ WIADD 291_ YLRTF 296_ LTQDG 301_ VSRLR 306_ NLRPD 311_ DVFII 316_ DDADE 321_ IPARD 326_ GVLFL 331_ KLYDG 336_ WTEPF 341_ AFHMR 346_ KSLYG 351_ FFWKQ 356_ PGTLE 361_ VVSGC 366_ TVDML 371_ QAVYG 376_ LDGIR 381_ LRRRQ 386_ YYTMP 391_ NFRQY 396_ ENRTG 401_ HILVQ 406_ WSLGS 411_ PLHFA 416_ GWHCS 421_ WCFTP 426_ EGIYF 431_ KLVSA 436_ QNGDF 441_ PRWGD 446_ YEDKR 451_ DLNYI 456_ RGLIR 461_ TGGWF 466_ DGTQQ 471_ EYPPA 476_ DPSEH 481_ MYAPK 486_ YLLKN 491_ YDRFH 496_ YLLDN 501_ PYQEP 506_ RSTAA 511_ GGWRH 516_ RGPEG 521_ RPPAR 526_GKLDE
1: It is involved in the regulation of the biosynthesis and biological function of glycoprotein oligosaccharides. Catalyzes the addition of N-acetylglucosamine in beta 1-4 linkage to the beta-linked mannose of the trimannosyl core of N-linked sugar chains, called bisecting N-acetylglucosamine (GlcNAc). It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. The addition of this bisecting GlcNAc residue alters not only the composition, but also the conformation of the N-glycan. The introduction of the bisecting GlcNAc residue results in the suppression of further processing and elongation of N-glycans, precluding the formation of beta-1,6 GlcNAc branching, catalyzed by MGAT5 since it is unable to use the bisected oligosaccharide as a substrate (PubMed:19403558). Addition of bisecting N-acetylglucosamine to CDH1/E-cadherin modulates CDH1 cell membrane location (PubMed:19403558). Inhibits NeuAc-alpha-2,3-Gal-beta-1,4-GlcNAc- formation which modulates sialylation levels and plays a role in cell migration regulation (PubMed:26801611). In brain, addition of bisecting N-acetylglucosamine to BACE1 blocks its lysosomal targeting in response to oxidative stress and further degradation which increases its location to early endosome and the APP cleavage (By similarity)