Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A (EC 2.4.1.155) (Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase V) (GlcNAc-T V) (GNT-V) (Mannoside acetylglucosaminyltransferase 5) (N-acetylglucosaminyl-transferase V) [Cleaved into: Secreted alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A (Secreted beta-1,6-N-acetylglucosaminyltransferase V) (Secreted GNT-V)]
1_MALFT 6_ PWKLS 11_ SQKLG 16_ FFLVT 21_ FGFIW 26_ GMMLL 31_ HFTIQ 36_ QRTQP 41_ ESSSM 46_ LREQI 51_ LDLSK 56_ RYIKA 61_ LAEEN 66_ RNVVD 71_ GPYAG 76_ VMTAY 81_ DLKKT 86_ LAVLL 91_ DNILQ 96_ RIGKL 101_ ESKVD 106_ NLVVN 111_ GTGTN 116_ STNST 121_ TAVPS 126_ LVALE 131_ KINVA 136_ DIING 141_ AQEKC 146_ VLPPM 151_ DGYPH 156_ CEGKI 161_ KWMKD 166_ MWRSD 171_ PCYAD 176_ YGVDG 181_ STCSF 186_ FIYLS 191_ EVENW 196_ CPHLP 201_ WRAKN 206_ PYEEA 211_ DHNSL 216_ AEIRT 221_ DFNIL 226_ YSMMK 231_ KHEEF 236_ RWMRL 241_ RIRRM 246_ ADAWI 251_ QAIKS 256_ LAEKQ 261_ NLEKR 266_ KRKKV 271_ LVHLG 276_ LLTKE 281_ SGFKI 286_ AETAF 291_ SGGPL 296_ GELVQ 301_ WSDLI 306_ TSLYL 311_ LGHDI 316_ RISAS 321_ LAELK 326_ EIMKK 331_ VVGNR 336_ SGCPT 341_ VGDRI 346_ VELIY 351_ IDIVG 356_ LAQFK 361_ KTLGP 366_ SWVHY 371_ QCMLR 376_ VLDSF 381_ GTEPE 386_ FNHAN 391_ YAQSK 396_ GHKTP 401_ WGKWN 406_ LNPQQ 411_ FYTMF 416_ PHTPD 421_ NSFLG 426_ FVVEQ 431_ HLNSS 436_ DIHHI 441_ NEIKR 446_ QNQSL 451_ VYGKV 456_ DSFWK 461_ NKKIY 466_ LDIIH 471_ TYMEV 476_ HATVY 481_ GSSTK 486_ NIPSY 491_ VKNHG 496_ ILSGR 501_ DLQFL 506_ LRETK 511_ LFVGL 516_ GFPYE 521_ GPAPL 526_ EAIAN 531_ GCAFL 536_ NPKFN 541_ PPKSS 546_ KNTDF 551_ FIGKP 556_ TLREL 561_ TSQHP 566_ YAEVF 571_ IGRPH 576_ VWTVD 581_ LNNQE 586_ EVEDA 591_ VKAIL 596_ NQKIE 601_ PYMPY 606_ EFTCE 611_ GMLQR 616_ INAFI 621_ EKQDF 626_ CHGQV 631_ MWPPL 636_ SALQV 641_ KLAEP 646_ GQSCK 651_ QVCQE 656_ SQLIC 661_ EPSFF 666_ QHLNK 671_ DKDML 676_ KYKVT 681_ CQSSE 686_ LAKDI 691_ LVPSF 696_ DPKNK 701_ HCVFQ 706_ GDLLL 711_ FSCAG 716_ AHPRH 721_ QRVCP 726_ CRDFI 731_ KGQVA 736_LCKDC
1: Catalyzes the addition of N-acetylglucosamine (GlcNAc) in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides (PubMed:10395745, PubMed:30140003). Catalyzes an important step in the biosynthesis of branched, complex-type N-glycans, such as those found on EGFR, TGFR (TGF-beta receptor) and CDH2 (PubMed:10395745, PubMed:22614033, PubMed:30140003). Via its role in the biosynthesis of complex N-glycans, plays an important role in the activation of cellular signaling pathways, reorganization of the actin cytoskeleton, cell-cell adhesion and cell migration. MGAT5-dependent EGFR N-glycosylation enhances the interaction between EGFR and LGALS3 and thereby prevents rapid EGFR endocytosis and prolongs EGFR signaling. Required for efficient interaction between TGFB1 and its receptor. Enhances activation of intracellular signaling pathways by several types of growth factors, including FGF2, PDGF, IGF, TGFB1 and EGF. MGAT5-dependent CDH2 N-glycosylation inhibits CDH2-mediated homotypic cell-cell adhesion and contributes to the regulation of downstream signaling pathways. Promotes cell migration. Contributes to the regulation of the inflammatory response. MGAT5-dependent TCR N-glycosylation enhances the interaction between TCR and LGALS3, limits agonist-induced TCR clustering, and thereby dampens TCR-mediated responses to antigens. Required for normal leukocyte evasation and accumulation at sites of inflammation (By similarity). Inhibits attachment of monocytes to the vascular endothelium and subsequent monocyte diapedesis (PubMed:22614033)
2: Promotes proliferation of umbilical vein endothelial cells and angiogenesis, at least in part by promoting the release of the growth factor FGF2 from the extracellular matrix