E3 ubiquitin-protein ligase TRIM32 (EC 2.3.2.27) (72 kDa Tat-interacting protein) (RING-type E3 ubiquitin transferase TRIM32) (Tripartite motif-containing protein 32) (Zinc finger protein HT2A)
1_MAAAA 6_ ASHLN 11_ LDALR 16_ EVLEC 21_ PICME 26_ SFTEE 31_ QLRPK 36_ LLHCG 41_ HTICR 46_ QCLEK 51_ LLASS 56_ INGVR 61_ CPFCS 66_ KITRI 71_ TSLTQ 76_ LTDNL 81_ TVLKI 86_ IDTAG 91_ LSEAV 96_ GLLMC 101_ RSCGR 106_ RLPRQ 111_ FCRSC 116_ GLVLC 121_ EPCRE 126_ ADHQP 131_ PGHCT 136_ LPVKE 141_ AAEER 146_ RRDFG 151_ EKLTR 156_ LRELM 161_ GELQR 166_ RKAAL 171_ EGVSK 176_ DLQAR 181_ YKAVL 186_ QEYGH 191_ EERRV 196_ QDELA 201_ RSRKF 206_ FTGSL 211_ AEVEK 216_ SNSQV 221_ VEEQS 226_ YLLNI 231_ AEVQA 236_ VSRCD 241_ YFLAK 246_ IKQAD 251_ VALLE 256_ ETADE 261_ EEPEL 266_ TASLP 271_ RELTL 276_ QDVEL 281_ LKVGH 286_ VGPLQ 291_ IGQAV 296_ KKPRT 301_ VNVED 306_ SWAME 311_ ATASA 316_ ASTSV 321_ TFREM 326_ DMSPE 331_ EVVAS 336_ PRASP 341_ AKQRG 346_ PEAAS 351_ NIQQC 356_ LFLKK 361_ MGAKG 366_ STPGM 371_ FNLPV 376_ SLYVT 381_ SQGEV 386_ LVADR 391_ GNYRI 396_ QVFTR 401_ KGFLK 406_ EIRRS 411_ PSGID 416_ SFVLS 421_ FLGAD 426_ LPNLT 431_ PLSVA 436_ MNCQG 441_ LIGVT 446_ DSYDN 451_ SLKVY 456_ TLDGH 461_ CVACH 466_ RSQLS 471_ KPWGI 476_ TALPS 481_ GQFVV 486_ TDVEG 491_ GKLWC 496_ FTVDR 501_ GSGVV 506_ KYSCL 511_ CSAVR 516_ PKFVT 521_ CDAEG 526_ TVYFT 531_ QGLGL 536_ NLENR 541_ QNEHH 546_ LEGGF 551_ SIGSV 556_ GPDGQ 561_ LGRQI 566_ SHFFS 571_ ENEDF 576_ RCIAG 581_ MCVDA 586_ RGDLI 591_ VADSS 596_ RKEIL 601_ HFPKG 606_ GGYSV 611_ LIREG 616_ LTCPV 621_ GIALT 626_ PKGQL 631_ LVLDC 636_ WDHCI 641_ KIYSY 646_HLRRY
1: E3 ubiquitin ligase that plays a role in various biological processes including neural stem cell differentiation, innate immunity, inflammatory resonse and autophagy (PubMed:19349376, PubMed:31123703). Plays a role in virus-triggered induction of IFN-beta and TNF-alpha by mediating the ubiquitination of STING1. Mechanistically, targets STING1 for 'Lys-63'-linked ubiquitination which promotes the interaction of STING1 with TBK1 (PubMed:22745133). Regulates bacterial clearance and promotes autophagy in Mycobacterium tuberculosis-infected macrophages (PubMed:37543647). Negatively regulates TLR3/4-mediated innate immune and inflammatory response by triggering the autophagic degradation of TICAM1 in an E3 activity-independent manner (PubMed:28898289). Plays an essential role in oxidative stress induced cell death by inducing loss of transmembrane potential and enhancing mitochondrial reactive oxygen species (ROS) production during oxidative stress conditions (PubMed:32918979). Ubiquitinates XIAP and targets it for proteasomal degradation (PubMed:21628460). Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation (PubMed:19349376). May ubiquitinate BBS2 (PubMed:22500027). Ubiquitinates PIAS4/PIASY and promotes its degradation in keratinocytes treated with UVB and TNF-alpha (By similarity). Also acts as a regulator of autophagy by mediating formation of unanchored 'Lys-63'-linked polyubiquitin chains that activate ULK1: interaction with AMBRA1 is required for ULK1 activation (PubMed:31123703). Positively regulates dendritic branching by promoting ubiquitination and subsequent degradation of the epigenetic factor CDYL (PubMed:34888944). Under metabolic stress and phosphorylation by CHK2, mediates 'Lys-63'-linked ubiquitination of ATG7 at 'Lys-45' to initiate autophagy (PubMed:37943659)
2: (Microbial infection) May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo (PubMed:7778269). Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo (PubMed:7778269)