Mitotic spindle assembly checkpoint protein MAD2A (HsMAD2) (Mitotic arrest deficient 2-like protein 1) (MAD2-like protein 1)
1_MALQL 6_ SREQG 11_ ITLRG 16_ SAEIV 21_ AEFFS 26_ FGINS 31_ ILYQR 36_ GIYPS 41_ ETFTR 46_ VQKYG 51_ LTLLV 56_ TTDLE 61_ LIKYL 66_ NNVVE 71_ QLKDW 76_ LYKCS 81_ VQKLV 86_ VVISN 91_ IESGE 96_ VLERW 101_ QFDIE 106_ CDKTA 111_ KDDSA 116_ PREKS 121_ QKAIQ 126_ DEIRS 131_ VIRQI 136_ TATVT 141_ FLPLL 146_ EVSCS 151_ FDLLI 156_ YTDKD 161_ LVVPE 166_ KWEES 171_ GPQFI 176_ TNSEE 181_ VRLRS 186_ FTTTI 191_ HKVNS 196_MVAYK
1: Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate (PubMed:15024386, PubMed:29162720). In the closed conformation (C-MAD2) forms a heterotetrameric complex with MAD1L1 at unattached kinetochores during prometaphase, the complex recruits open conformation molecules of MAD2L1 (O-MAD2) and then promotes the conversion of O-MAD2 to C-MAD2 (PubMed:29162720). Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate (PubMed:10700282, PubMed:11804586, PubMed:15024386)