DNA topoisomerase 3-alpha (EC 5.6.2.1) (DNA topoisomerase III alpha)
1_MIFPV 6_ ARYAL 11_ RWLRR 16_ PEDRA 21_ FSRAA 26_ MEMAL 31_ RGVRK 36_ VLCVA 41_ EKNDA 46_ AKGIA 51_ DLLSN 56_ GRMRR 61_ REGLS 66_ KFNKI 71_ YEFDY 76_ HLYGQ 81_ NVTMV 86_ MTSVS 91_ GHLLA 96_ HDFQM 101_ QFRKW 106_ QSCNP 111_ LVLFE 116_ AEIEK 121_ YCPEN 126_ FVDIK 131_ KTLER 136_ ETRQC 141_ QALVI 146_ WTDCD 151_ REGEN 156_ IGFEI 161_ IHVCK 166_ AVKPN 171_ LQVLR 176_ ARFSE 181_ ITPHA 186_ VRTAC 191_ ENLTE 196_ PDQRV 201_ SDAVD 206_ VRQEL 211_ DLRIG 216_ AAFTR 221_ FQTLR 226_ LQRIF 231_ PEVLA 236_ EQLIS 241_ YGSCQ 246_ FPTLG 251_ FVVER 256_ FKAIQ 261_ AFVPE 266_ IFHRI 271_ KVTHD 276_ HKDGI 281_ VEFNW 286_ KRHRL 291_ FNHTA 296_ CLVLY 301_ QLCVE 306_ DPMAT 311_ VVEVR 316_ SKPKS 321_ KWRPQ 326_ ALDTV 331_ ELEKL 336_ ASRKL 341_ RINAK 346_ ETMRI 351_ AEKLY 356_ TQGYI 361_ SYPRT 366_ ETNIF 371_ PRDLN 376_ LTVLV 381_ EQQTP 386_ DPRWG 391_ AFAQS 396_ ILERG 401_ GPTPR 406_ NGNKS 411_ DQAHP 416_ PIHPT 421_ KYTNN 426_ LQGDE 431_ QRLYE 436_ FIVRH 441_ FLACC 446_ SQDAQ 451_ GQETT 456_ VEIDI 461_ AQERF 466_ VAHGL 471_ MILAR 476_ NYLDV 481_ YPYDH 486_ WSDKI 491_ LPVYE 496_ QGSHF 501_ QPSTV 506_ EMVDG 511_ ETSPP 516_ KLLTE 521_ ADLIA 526_ LMEKH 531_ GIGTD 536_ ATHAE 541_ HIETI 546_ KARMY 551_ VGLTP 556_ DKRFL 561_ PGHLG 566_ MGLVE 571_ GYDSM 576_ GYEMS 581_ KPDLR 586_ AELEA 591_ DLKLI 596_ CDGKK 601_ DKFVV 606_ LRQQV 611_ QKYKQ 616_ VFIEA 621_ VAKAK 626_ KLDEA 631_ LAQYF 636_ GNGTE 641_ LAQQE 646_ DIYPA 651_ MPEPI 656_ RKCPQ 661_ CNKDM 666_ VLKTK 671_ KNGGF 676_ YLSCM 681_ GFPEC 686_ RSAVW 691_ LPDSV 696_ LEASR 701_ DSSVC 706_ PVCQP 711_ HPVYR 716_ LKLKF 721_ KRGSL 726_ PPTMP 731_ LEFVC 736_ CIGGC 741_ DDTLR 746_ EILDL 751_ RFSGG 756_ PPRAS 761_ QPSGR 766_ LQANQ 771_ SLNRM 776_ DNSQH 781_ PQPAD 786_ SRQTG 791_ SSKAL 796_ AQTLP 801_ PPTAA 806_ GESNS 811_ VTCNC 816_ GQEAV 821_ LLTVR 826_ KEGPN 831_ RGRQF 836_ FKCNG 841_ GSCNF 846_ FLWAD 851_ SPNPG 856_ AGGPP 861_ ALAYR 866_ PLGAS 871_ LGCPP 876_ GPGIH 881_ LGGFG 886_ NPGDG 891_ SGSGT 896_ SCLCS 901_ QPSVT 906_ RTVQK 911_ DGPNK 916_ GRQFH 921_ TCAKP 926_ REQQC 931_ GFFQW 936_ VDENT 941_ APGTS 946_ GAPSW 951_ TGDRG 956_ RTLES 961_ EARSK 966_ RPRAS 971_ SSDMG 976_ STAKK 981_ PRKCS 986_ LCHQP 991_ GHTRP 996_FCPQN
1: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. As an essential component of the RMI complex it is involved in chromosome separation and the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity (PubMed:30057030). It is required for mtDNA decatenation and segregation after completion of replication, in a process that does not require BLM, RMI1 and RMI2 (PubMed:29290614)