NEDD8-activating enzyme E1 regulatory subunit (Amyloid beta precursor protein-binding protein 1, 59 kDa) (APP-BP1) (Amyloid protein-binding protein 1) (Proto-oncogene protein 1)
1_MAQLG 6_ KLLKE 11_ QKYDR 16_ QLRLW 21_ GDHGQ 26_ EALES 31_ AHVCL 36_ INATA 41_ TGTEI 46_ LKNLV 51_ LPGIG 56_ SFTII 61_ DGNQV 66_ SGEDA 71_ GNNFF 76_ LQRSS 81_ IGKNR 86_ AEAAM 91_ EFLQE 96_ LNSDV 101_ SGSFV 106_ EESPE 111_ NLLDN 116_ DPSFF 121_ CRFTV 126_ VVATQ 131_ LPEST 136_ SLRLA 141_ DVLWN 146_ SQIPL 151_ LICRT 156_ YGLVG 161_ YMRII 166_ IKEHP 171_ VIESH 176_ PDNAL 181_ EDLRL 186_ DKPFP 191_ ELREH 196_ FQSYD 201_ LDHME 206_ KKDHS 211_ HTPWI 216_ VIIAK 221_ YLAQW 226_ YSETN 231_ GRIPK 236_ TYKEK 241_ EDFRD 246_ LIRQG 251_ ILKNE 256_ NGAPE 261_ DEENF 266_ EEAIK 271_ NVNTA 276_ LNTTQ 281_ IPSSI 286_ EDIFN 291_ DDRCI 296_ NITKQ 301_ TPSFW 306_ ILARA 311_ LKEFV 316_ AKEGQ 321_ GNLPV 326_ RGTIP 331_ DMIAD 336_ SGKYI 341_ KLQNV 346_ YREKA 351_ KKDAA 356_ AVGNH 361_ VAKLL 366_ QSIGQ 371_ APESI 376_ SEKEL 381_ KLLCS 386_ NSAFL 391_ RVVRC 396_ RSLAE 401_ EYGLD 406_ TINKD 411_ EIISS 416_ MDNPD 421_ NEIVL 426_ YLMLR 431_ AVDRF 436_ HKQQG 441_ RYPGV 446_ SNYQV 451_ EEDIG 456_ KLKSC 461_ LTGFL 466_ QEYGL 471_ SVMVK 476_ DDYVH 481_ EFCRY 486_ GAAEP 491_ HTIAA 496_ FLGGA 501_ AAQEV 506_ IKIIT 511_ KQFVI 516_ FNNTY 521_ IYSGM 526_SQTSA
1: Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. The covalent attachment of NEDD8 to target proteins is known as 'neddylation' and the process is involved in the regulation of cell growth, viability and development