Nucleic acid dioxygenase ALKBH1 (EC 1.14.11.-) (Alkylated DNA repair protein alkB homolog 1) (Alpha-ketoglutarate-dependent dioxygenase ABH1) (DNA 6mA demethylase) (DNA N6-methyl adenine demethylase ALKBH1) (EC 1.14.11.51) (DNA lyase ABH1) (EC 4.2.99.18) (DNA oxidative demethylase ALKBH1) (EC 1.14.11.33) (mRNA N(3)-methylcytidine demethylase) (EC 1.14.11.-)
1_MGKMA 6_ AAVGS 11_ VATLA 16_ TEPGE 21_ DAFRK 26_ LFRFY 31_ RQSRP 36_ GTADL 41_ EGVID 46_ FSAAH 51_ AARGK 56_ GPGAQ 61_ KVIKS 66_ QLNVS 71_ SVSEQ 76_ NAYRA 81_ GLQPV 86_ SKWQA 91_ YGLKG 96_ YPGFI 101_ FIPNP 106_ FLPGY 111_ QWHWV 116_ KQCLK 121_ LYSQK 126_ PNVCN 131_ LDKHM 136_ SKEET 141_ QDLWE 146_ QSKEF 151_ LRYKE 156_ ATKRR 161_ PRSLL 166_ EKLRW 171_ VTVGY 176_ HYNWD 181_ SKKYS 186_ ADHYT 191_ PFPSD 196_ LGFLS 201_ EQVAA 206_ ACGFE 211_ DFRAE 216_ AGILN 221_ YYRLD 226_ STLGI 231_ HVDRS 236_ ELDHS 241_ KPLLS 246_ FSFGQ 251_ SAIFL 256_ LGGLQ 261_ RDEAP 266_ TAMFM 271_ HSGDI 276_ MIMSG 281_ FSRLL 286_ NHAVP 291_ RVLPN 296_ PEGEG 301_ LPHCL 306_ EAPLP 311_ AVLPR 316_ DSMVE 321_ PCSME 326_ DWQVC 331_ ASYLK 336_ TARVN 341_ MTVRQ 346_ VLATD 351_ QNFPL 356_ EPIED 361_ EKRDI 366_ STEGF 371_ CHLDD 376_ QNSEV 381_KRARI
1: Dioxygenase that acts on nucleic acids, such as DNA and tRNA (PubMed:18603530, PubMed:27497299, PubMed:27745969). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:18603530, PubMed:27497299). A number of activities have been described for this dioxygenase, but recent results suggest that it mainly acts on tRNAs and mediates their demethylation or oxidation depending on the context and subcellular compartment (PubMed:27497299, PubMed:27745969). Mainly acts as a tRNA demethylase by removing N(1)-methyladenine from various tRNAs, with a preference for N(1)-methyladenine at position 58 (m1A58) present on a stem loop structure of tRNAs (PubMed:27745969). Acts as a regulator of translation initiation and elongation in response to glucose deprivation: regulates both translation initiation, by mediating demethylation of tRNA(Met), and translation elongation, N(1)-methyladenine-containing tRNAs being preferentially recruited to polysomes to promote translation elongation (PubMed:27745969). In mitochondrion, specifically interacts with mt-tRNA(Met) and mediates oxidation of mt-tRNA(Met) methylated at cytosine(34) to form 5-formylcytosine (f(5)c) at this position (PubMed:27497299). mt-tRNA(Met) containing the f(5)c modification at the wobble position enables recognition of the AUA codon in addition to the AUG codon, expanding codon recognition in mitochondrial translation (PubMed:27497299). Specifically demethylates DNA methylated on the 6th position of adenine (N(6)-methyladenosine) DNA (PubMed:30017583, PubMed:30392959). N(6)-methyladenosine (m6A) DNA is present at some L1 elements in embryonic stem cells and probably promotes their silencing (By similarity). Demethylates mRNAs containing N(3)-methylcytidine modification (PubMed:31188562). Also able to repair alkylated single-stranded DNA by oxidative demethylation, but with low activity (PubMed:18603530). Also has DNA lyase activity and introduces double-stranded breaks at abasic sites: cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites (PubMed:19959401). DNA lyase activity does not require alpha-ketoglutarate and iron and leads to the formation of an irreversible covalent protein-DNA adduct with the 5' DNA product (PubMed:19959401, PubMed:23577621). DNA lyase activity is not required during base excision repair and class switch recombination of the immunoglobulin heavy chain during B lymphocyte activation. May play a role in placental trophoblast lineage differentiation (By similarity)