Spliceosome RNA helicase DDX39B (EC 3.6.4.13) (56 kDa U2AF65-associated protein) (ATP-dependent RNA helicase p47) (DEAD box protein UAP56) (HLA-B-associated transcript 1 protein)
1_MAEND 6_ VDNEL 11_ LDYED 16_ DEVET 21_ AAGGD 26_ GAEAP 31_ AKKDV 36_ KGSYV 41_ SIHSS 46_ GFRDF 51_ LLKPE 56_ LLRAI 61_ VDCGF 66_ EHPSE 71_ VQHEC 76_ IPQAI 81_ LGMDV 86_ LCQAK 91_ SGMGK 96_ TAVFV 101_ LATLQ 106_ QLEPV 111_ TGQVS 116_ VLVMC 121_ HTREL 126_ AFQIS 131_ KEYER 136_ FSKYM 141_ PNVKV 146_ AVFFG 151_ GLSIK 156_ KDEEV 161_ LKKNC 166_ PHIVV 171_ GTPGR 176_ ILALA 181_ RNKSL 186_ NLKHI 191_ KHFIL 196_ DECDK 201_ MLEQL 206_ DMRRD 211_ VQEIF 216_ RMTPH 221_ EKQVM 226_ MFSAT 231_ LSKEI 236_ RPVCR 241_ KFMQD 246_ PMEIF 251_ VDDET 256_ KLTLH 261_ GLQQY 266_ YVKLK 271_ DNEKN 276_ RKLFD 281_ LLDVL 286_ EFNQV 291_ VIFVK 296_ SVQRC 301_ IALAQ 306_ LLVEQ 311_ NFPAI 316_ AIHRG 321_ MPQEE 326_ RLSRY 331_ QQFKD 336_ FQRRI 341_ LVATN 346_ LFGRG 351_ MDIER 356_ VNIAF 361_ NYDMP 366_ EDSDT 371_ YLHRV 376_ ARAGR 381_ FGTKG 386_ LAITF 391_ VSDEN 396_ DAKIL 401_ NDVQD 406_ RFEVN 411_ ISELP 416_ DEIDI 421_SSYIE
1: Involved in nuclear export of spliced and unspliced mRNA (PubMed:15833825, PubMed:15998806, PubMed:17190602). Component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA (PubMed:15833825, PubMed:15998806, PubMed:17190602). The TREX complex is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NXF1 pathway (PubMed:15833825, PubMed:15998806, PubMed:17190602). The THOC1-THOC2-THOC3 core complex alone is sufficient to promote ATPase activity of DDX39B; in the complex THOC2 is the only component that directly interacts with DDX39B (PubMed:33191911). Associates with SARNP/CIP29, which facilitates RNA binding of DDX39B and likely plays a role in mRNA export (PubMed:37578863). May undergo several rounds of ATP hydrolysis during assembly of TREX to drive subsequent loading of components such as ALYREF/THOC4 and CHTOP onto mRNA. Also associates with pre-mRNA independent of ALYREF/THOC4. Involved in the nuclear export of intronless mRNA; the ATP-bound form is proposed to recruit export adapter ALYREF/THOC4 to intronless mRNA; its ATPase activity is cooperatively stimulated by RNA and ALYREF/THOC4 and ATP hydrolysis is thought to trigger the dissociation from RNA to allow the association of ALYREF/THOC4 and the NXF1-NXT1 heterodimer. Involved in transcription elongation and genome stability
2: Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. Has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. Can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. Can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2; the effect of ALYREF/THOC4 is reported conflictingly with [PubMed:23299939] reporting a stimulatory effect
3: (Microbial infection) The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production