Werner syndrome ATP-dependent helicase (EC 3.6.4.12) (DNA helicase, RecQ-like type 3) (RecQ3) (Exonuclease WRN) (EC 3.1.-.-) (RecQ protein-like 2)
1_MSEKK 6_ LETTA 11_ QQRKC 16_ PEWMN 21_ VQNKR 26_ CAVEE 31_ RKACV 36_ RKSVF 41_ EDDLP 46_ FLEFT 51_ GSIVY 56_ SYDAS 61_ DCSFL 66_ SEDIS 71_ MSLSD 76_ GDVVG 81_ FDMEW 86_ PPLYN 91_ RGKLG 96_ KVALI 101_ QLCVS 106_ ESKCY 111_ LFHVS 116_ SMSVF 121_ PQGLK 126_ MLLEN 131_ KAVKK 136_ AGVGI 141_ EGDQW 146_ KLLRD 151_ FDIKL 156_ KNFVE 161_ LTDVA 166_ NKKLK 171_ CTETW 176_ SLNSL 181_ VKHLL 186_ GKQLL 191_ KDKSI 196_ RCSNW 201_ SKFPL 206_ TEDQK 211_ LYAAT 216_ DAYAG 221_ FIIYR 226_ NLEIL 231_ DDTVQ 236_ RFAIN 241_ KEEEI 246_ LLSDM 251_ NKQLT 256_ SISEE 261_ VMDLA 266_ KHLPH 271_ AFSKL 276_ ENPRR 281_ VSILL 286_ KDISE 291_ NLYSL 296_ RRMII 301_ GSTNI 306_ ETELR 311_ PSNNL 316_ NLLSF 321_ EDSTT 326_ GGVQQ 331_ KQIRE 336_ HEVLI 341_ HVEDE 346_ TWDPT 351_ LDHLA 356_ KHDGE 361_ DVLGN 366_ KVERK 371_ EDGFE 376_ DGVED 381_ NKLKE 386_ NMERA 391_ CLMSL 396_ DITEH 401_ ELQIL 406_ EQQSQ 411_ EEYLS 416_ DIAYK 421_ STEHL 426_ SPNDN 431_ ENDTS 436_ YVIES 441_ DEDLE 446_ MEMLK 451_ HLSPN 456_ DNEND 461_ TSYVI 466_ ESDED 471_ LEMEM 476_ LKSLE 481_ NLNSG 486_ TVEPT 491_ HSKCL 496_ KMERN 501_ LGLPT 506_ KEEEE 511_ DDENE 516_ ANEGE 521_ EDDDK 526_ DFLWP 531_ APNEE 536_ QVTCL 541_ KMYFG 546_ HSSFK 551_ PVQWK 556_ VIHSV 561_ LEERR 566_ DNVAV 571_ MATGY 576_ GKSLC 581_ FQYPP 586_ VYVGK 591_ IGLVI 596_ SPLIS 601_ LMEDQ 606_ VLQLK 611_ MSNIP 616_ ACFLG 621_ SAQSE 626_ NVLTD 631_ IKLGK 636_ YRIVY 641_ VTPEY 646_ CSGNM 651_ GLLQQ 656_ LEADI 661_ GITLI 666_ AVDEA 671_ HCISE 676_ WGHDF 681_ RDSFR 686_ KLGSL 691_ KTALP 696_ MVPIV 701_ ALTAT 706_ ASSSI 711_ REDIV 716_ RCLNL 721_ RNPQI 726_ TCTGF 731_ DRPNL 736_ YLEVR 741_ RKTGN 746_ ILQDL 751_ QPFLV 756_ KTSSH 761_ WEFEG 766_ PTIIY 771_ CPSRK 776_ MTQQV 781_ TGELR 786_ KLNLS 791_ CGTYH 796_ AGMSF 801_ STRKD 806_ IHHRF 811_ VRDEI 816_ QCVIA 821_ TIAFG 826_ MGINK 831_ ADIRQ 836_ VIHYG 841_ APKDM 846_ ESYYQ 851_ EIGRA 856_ GRDGL 861_ QSSCH 866_ VLWAP 871_ ADINL 876_ NRHLL 881_ TEIRN 886_ EKFRL 891_ YKLKM 896_ MAKME 901_ KYLHS 906_ SRCRR 911_ QIILS 916_ HFEDK 921_ QVQKA 926_ SLGIM 931_ GTEKC 936_ CDNCR 941_ SRLDH 946_ CYSMD 951_ DSEDT 956_ SWDFG 961_ PQAFK 966_ LLSAV 971_ DILGE 976_ KFGIG 981_ LPILF 986_ LRGSN 991_ SQRLA 996_ DQYRR 1001_ HSLFG 1006_ TGKDQ 1011_ TESWW 1016_ KAFSR 1021_ QLITE 1026_ GFLVE 1031_ VSRYN 1036_ KFMKI 1041_ CALTK 1046_ KGRNW 1051_ LHKAN 1056_ TESQS 1061_ LILQA 1066_ NEELC 1071_ PKKLL 1076_ LPSSK 1081_ TVSSG 1086_ TKEHC 1091_ YNQVP 1096_ VELST 1101_ EKKSN 1106_ LEKLY 1111_ SYKPC 1116_ DKISS 1121_ GSNIS 1126_ KKSIM 1131_ VQSPE 1136_ KAYSS 1141_ SQPVI 1146_ SAQEQ 1151_ ETQIV 1156_ LYGKL 1161_ VEARQ 1166_ KHANK 1171_ MDVPP 1176_ AILAT 1181_ NKILV 1186_ DMAKM 1191_ RPTTV 1196_ ENVKR 1201_ IDGVS 1206_ EGKAA 1211_ MLAPL 1216_ LEVIK 1221_ HFCQT 1226_ NSVQT 1231_ DLFSS 1236_ TKPQE 1241_ EQKTS 1246_ LVAKN 1251_ KICTL 1256_ SQSMA 1261_ ITYSL 1266_ FQEKK 1271_ MPLKS 1276_ IAESR 1281_ ILPLM 1286_ TIGMH 1291_ LSQAV 1296_ KAGCP 1301_ LDLER 1306_ AGLTP 1311_ EVQKI 1316_ IADVI 1321_ RNPPV 1326_ NSDMS 1331_ KISLI 1336_ RMLVP 1341_ ENIDT 1346_ YLIHM 1351_ AIEIL 1356_ KHGPD 1361_ SGLQP 1366_ SCDVN 1371_ KRRCF 1376_ PGSEE 1381_ ICSSS 1386_ KRSKE 1391_ EVGIN 1396_ TETSS 1401_ AERKR 1406_ RLPVW 1411_ FAKGS 1416_ DTSKK 1421_ LMDKT 1426_KRGGL
1: Multifunctional enzyme that has magnesium and ATP-dependent 3'-5' DNA-helicase activity on partially duplex substrates (PubMed:9224595, PubMed:9288107, PubMed:9611231). Also has 3'->5' exonuclease activity towards double-stranded (ds)DNA with a 5'-overhang (PubMed:11863428). Has no nuclease activity towards single-stranded (ss)DNA or blunt-ended dsDNA (PubMed:11863428). Helicase activity is most efficient with (d)ATP, but (d)CTP will substitute with reduced efficiency; strand displacement is enhanced by single-strand binding-protein (heterotrimeric replication protein A complex, RPA1, RPA2, RPA3) (PubMed:9611231). Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity). Plays a role in double-strand break repair after gamma-irradiation (PubMed:9224595, PubMed:9288107, PubMed:9611231). Unwinds some G-quadruplex DNA (d(CGG)n tracts); unwinding seems to occur in both 5'-3' and 3'-5' direction and requires a short single-stranded tail (PubMed:10212265). d(CGG)n tracts have a propensity to assemble into tetraplex structures; other G-rich substrates from a telomeric or IgG switch sequence are not unwound (PubMed:10212265). Depletion leads to chromosomal breaks and genome instability (PubMed:33199508)