Endonuclease G, mitochondrial (Endo G) (EC 3.1.30.-)
1_MRALR 6_ AGLTL 11_ ASGAG 16_ LGAVV 21_ EGWRR 26_ RREDA 31_ RAAPG 36_ LLGRL 41_ PVLPV 46_ AAAAE 51_ LPPVP 56_ GGPRG 61_ PGELA 66_ KYGLP 71_ GLAQL 76_ KSRES 81_ YVLCY 86_ DPRTR 91_ GALWV 96_ VEQLR 101_ PERLR 106_ GDGDR 111_ RECDF 116_ REDDS 121_ VHAYH 126_ RATNA 131_ DYRGS 136_ GFDRG 141_ HLAAA 146_ ANHRW 151_ SQKAM 156_ DDTFY 161_ LSNVA 166_ PQVPH 171_ LNQNA 176_ WNNLE 181_ KYSRS 186_ LTRSY 191_ QNVYV 196_ CTGPL 201_ FLPRT 206_ EADGK 211_ SYVKY 216_ QVIGK 221_ NHVAV 226_ PTHFF 231_ KVLIL 236_ EAAGG 241_ QIELR 246_ TYVMP 251_ NAPVD 256_ EAIPL 261_ ERFLV 266_ PIESI 271_ ERASG 276_ LLFVP 281_ NILAR 286_ AGSLK 291_AITAG
1: Endonuclease that preferentially catalyzes the cleavage of double-stranded 5-hydroxymethylcytosine (5hmC)-modified DNA (PubMed:25355512). The 5hmC-modified nucleotide does not increase the binding affinity, but instead increases the efficiency of cutting and specifies the site of cleavage for the modified DNAs (By similarity). Shows significantly higher affinity for four-stranded Holliday junction over duplex and single-stranded DNAs (By similarity). Promotes conservative recombination when the DNA is 5hmC-modified (PubMed:25355512). Promotes autophagy through the suppression of mTOR by its phosphorylation-mediated interaction with YWHAG and its endonuclease activity-mediated DNA damage response (PubMed:33473107). GSK3-beta mediated phosphorylation of ENDOG enhances its interaction with YWHAG, leading to the release of TSC2 and PIK3C3 from YWHAG resulting in mTOR pathway suppression and autophagy initiation (PubMed:33473107). Promotes cleavage of mtDNA in response to oxidative and nitrosative stress, in turn inducing compensatory mtDNA replication (PubMed:29719607)