Methylsterol monooxygenase 1 (EC 1.14.18.9) (C-4 methylsterol oxidase) (Sterol-C4-methyl oxidase)
1_MATNE 6_ SVSIF 11_ SSASL 16_ AVEYV 21_ DSLLP 26_ ENPLQ 31_ EPFKN 36_ AWNYM 41_ LNNYT 46_ KFQIA 51_ TWGSL 56_ IVHEA 61_ LYFLF 66_ CLPGF 71_ LFQFI 76_ PYMKK 81_ YKIQK 86_ DKPET 91_ WENQW 96_ KCFKV 101_ LLFNH 106_ FCIQL 111_ PLICG 116_ TYYFT 121_ EYFNI 126_ PYDWE 131_ RMPRW 136_ YFLLA 141_ RCFGC 146_ AVIED 151_ TWHYF 156_ LHRLL 161_ HHKRI 166_ YKYIH 171_ KVHHE 176_ FQAPF 181_ GMEAE 186_ YAHPL 191_ ETLIL 196_ GTGFF 201_ IGIVL 206_ LCDHV 211_ ILLWA 216_ WVTIR 221_ LLETI 226_ DVHSG 231_ YDIPL 236_ NPLNL 241_ IPFYA 246_ GSRHH 251_ DFHHM 256_ NFIGN 261_ YASTF 266_ TWWDR 271_ IFGTD 276_ SQYNA 281_ YNEKR 286_KKFEK
1: Catalyzes the three-step monooxygenation required for the demethylation of 4,4-dimethyl and 4alpha-methylsterols, which can be subsequently metabolized to cholesterol (PubMed:21285510, PubMed:23583456, PubMed:26114596, PubMed:28673550, PubMed:36958722). Also involved in drug metabolism, as it can metabolize eldecalcitol (ED-71 or 1alpha,25-dihydroxy-2beta-(3-hydroxypropoxy)-cholecalciferol), a second-generation vitamin D analog, into 1alpha,2beta,25-trihydroxy vitamin D3; this reaction occurs via enzymatic hydroxylation and spontaneous O-dehydroxypropylation (PubMed:26038696)