Cytochrome P450 1B1 (EC 1.14.14.1) (CYPIB1) (Hydroperoxy icosatetraenoate dehydratase) (EC 4.2.1.152)
1_MGTSL 6_ SPNDP 11_ WPLNP 16_ LSIQQ 21_ TTLLL 26_ LLSVL 31_ ATVHV 36_ GQRLL 41_ RQRRR 46_ QLRSA 51_ PPGPF 56_ AWPLI 61_ GNAAA 66_ VGQAA 71_ HLSFA 76_ RLARR 81_ YGDVF 86_ QIRLG 91_ SCPIV 96_ VLNGE 101_ RAIHQ 106_ ALVQQ 111_ GSAFA 116_ DRPAF 121_ ASFRV 126_ VSGGR 131_ SMAFG 136_ HYSEH 141_ WKVQR 146_ RAAHS 151_ MMRNF 156_ FTRQP 161_ RSRQV 166_ LEGHV 171_ LSEAR 176_ ELVAL 181_ LVRGS 186_ ADGAF 191_ LDPRP 196_ LTVVA 201_ VANVM 206_ SAVCF 211_ GCRYS 216_ HDDPE 221_ FRELL 226_ SHNEE 231_ FGRTV 236_ GAGSL 241_ VDVMP 246_ WLQYF 251_ PNPVR 256_ TVFRE 261_ FEQLN 266_ RNFSN 271_ FILDK 276_ FLRHC 281_ ESLRP 286_ GAAPR 291_ DMMDA 296_ FILSA 301_ EKKAA 306_ GDSHG 311_ GGARL 316_ DLENV 321_ PATIT 326_ DIFGA 331_ SQDTL 336_ STALQ 341_ WLLLL 346_ FTRYP 351_ DVQTR 356_ VQAEL 361_ DQVVG 366_ RDRLP 371_ CMGDQ 376_ PNLPY 381_ VLAFL 386_ YEAMR 391_ FSSFV 396_ PVTIP 401_ HATTA 406_ NTSVL 411_ GYHIP 416_ KDTVV 421_ FVNQW 426_ SVNHD 431_ PLKWP 436_ NPENF 441_ DPARF 446_ LDKDG 451_ LINKD 456_ LTSRV 461_ MIFSV 466_ GKRRC 471_ IGEEL 476_ SKMQL 481_ FLFIS 486_ ILAHQ 491_ CDFRA 496_ NPNEP 501_ AKMNF 506_ SYGLT 511_ IKPKS 516_ FKVNV 521_ TLRES 526_ MELLD 531_ SAVQN 536_LQAKE
1: A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins (PubMed:10681376, PubMed:11555828, PubMed:12865317, PubMed:15258110, PubMed:20972997). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:10681376, PubMed:11555828, PubMed:12865317, PubMed:15258110, PubMed:20972997). Exhibits catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2- and 4-hydroxy E1 and E2. Displays a predominant hydroxylase activity toward E2 at the C-4 position (PubMed:11555828, PubMed:12865317). Metabolizes testosterone and progesterone to B or D ring hydroxylated metabolites (PubMed:10426814). May act as a major enzyme for all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid (PubMed:10681376, PubMed:15258110). Catalyzes the epoxidation of double bonds of certain PUFA. Converts arachidonic acid toward epoxyeicosatrienoic acid (EpETrE) regioisomers, 8,9-, 11,12-, and 14,15- EpETrE, that function as lipid mediators in the vascular system (PubMed:20972997). Additionally, displays dehydratase activity toward oxygenated eicosanoids hydroperoxyeicosatetraenoates (HpETEs). This activity is independent of cytochrome P450 reductase, NADPH, and O2 (PubMed:21068195). Also involved in the oxidative metabolism of xenobiotics, particularly converting polycyclic aromatic hydrocarbons and heterocyclic aryl amines procarcinogens to DNA-damaging products (PubMed:10426814). Plays an important role in retinal vascular development. Under hyperoxic O2 conditions, promotes retinal angiogenesis and capillary morphogenesis, likely by metabolizing the oxygenated products generated during the oxidative stress. Also, contributes to oxidative homeostasis and ultrastructural organization and function of trabecular meshwork tissue through modulation of POSTN expression (By similarity)