Lanosterol 14-alpha demethylase (LDM) (EC 1.14.14.154) (CYPLI) (Cytochrome P450 51A1) (Cytochrome P450-14DM) (Cytochrome P45014DM) (Cytochrome P450LI) (Sterol 14-alpha demethylase)
1_MAAAA 6_ GMLLL 11_ GLLQA 16_ GGSVL 21_ GQAME 26_ KVTGG 31_ NLLSM 36_ LLIAC 41_ AFTLS 46_ LVYLI 51_ RLAAG 56_ HLVQL 61_ PAGVK 66_ SPPYI 71_ FSPIP 76_ FLGHA 81_ IAFGK 86_ SPIEF 91_ LENAY 96_ EKYGP 101_ VFSFT 106_ MVGKT 111_ FTYLL 116_ GSDAA 121_ ALLFN 126_ SKNED 131_ LNAED 136_ VYSRL 141_ TTPVF 146_ GKGVA 151_ YDVPN 156_ PVFLE 161_ QKKML 166_ KSGLN 171_ IAHFK 176_ QHVSI 181_ IEKET 186_ KEYFE 191_ SWGES 196_ GEKNV 201_ FEALS 206_ ELIIL 211_ TASHC 216_ LHGKE 221_ IRSQL 226_ NEKVA 231_ QLYAD 236_ LDGGF 241_ SHAAW 246_ LLPGW 251_ LPLPS 256_ FRRRD 261_ RAHRE 266_ IKDIF 271_ YKAIQ 276_ KRRQS 281_ QEKID 286_ DILQT 291_ LLDAT 296_ YKDGR 301_ PLTDD 306_ EVAGM 311_ LIGLL 316_ LAGQH 321_ TSSTT 326_ SAWMG 331_ FFLAR 336_ DKTLQ 341_ KKCYL 346_ EQKTV 351_ CGENL 356_ PPLTY 361_ DQLKD 366_ LNLLD 371_ RCIKE 376_ TLRLR 381_ PPIMI 386_ MMRMA 391_ RTPQT 396_ VAGYT 401_ IPPGH 406_ QVCVS 411_ PTVNQ 416_ RLKDS 421_ WVERL 426_ DFNPD 431_ RYLQD 436_ NPASG 441_ EKFAY 446_ VPFGA 451_ GRHRC 456_ IGENF 461_ AYVQI 466_ KTIWS 471_ TMLRL 476_ YEFDL 481_ IDGYF 486_ PTVNY 491_ TTMIH 496_ TPENP 501_VIRYK
1: Sterol 14alpha-demethylase that plays a critical role in the cholesterol biosynthesis pathway, being cholesterol the major sterol component in mammalian membranes as well as a precursor for bile acid and steroid hormone synthesis (PubMed:20149798, PubMed:8619637, PubMed:9559662). Cytochrome P450 monooxygenase that catalyzes the three-step oxidative removal of the 14alpha-methyl group (C-32) of sterols such as lanosterol (lanosta-8,24-dien-3beta-ol) and 24,25-dihydrolanosterol (DHL) in the form of formate, and converts the sterols to 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol and 4,4-dimethyl-8,14-cholestadien-3beta-ol, respectively, which are intermediates of cholesterol biosynthesis (PubMed:20149798, PubMed:8619637, PubMed:9559662). Can also demethylate substrates not intrinsic to mammals, such as eburicol (24-methylene-24,25-dihydrolanosterol), but at a lower rate than DHL (PubMed:9559662)