Flap endonuclease GEN homolog 1 (EC 3.1.-.-)
1_MGVND 6_ LWQIL 11_ EPVKQ 16_ HIPLR 21_ NLGGK 26_ TIAVD 31_ LSLWV 36_ CEAQT 41_ VKKMM 46_ GSVMK 51_ PHLRN 56_ LFFRI 61_ SYLTQ 66_ MDVKL 71_ VFVME 76_ GEPPK 81_ LKADV 86_ ISKRN 91_ QSRYG 96_ SSGKS 101_ WSQKT 106_ GRSHF 111_ KSVLR 116_ ECLHM 121_ LECLG 126_ IPWVQ 131_ AAGEA 136_ EAMCA 141_ YLNAG 146_ GHVDG 151_ CLTND 156_ GDTFL 161_ YGAQT 166_ VYRNF 171_ TMNTK 176_ DPHVD 181_ CYTMS 186_ SIKSK 191_ LGLDR 196_ DALVG 201_ LAILL 206_ GCDYL 211_ PKGVP 216_ GVGKE 221_ QALKL 226_ IQILK 231_ GQSLL 236_ QRFNR 241_ WNETS 246_ CNSSP 251_ QLLVT 256_ KKLAH 261_ CSVCS 266_ HPGSP 271_ KDHER 276_ NGCRL 281_ CKSDK 286_ YCEPH 291_ DYEYC 296_ CPCEW 301_ HRTEH 306_ DRQLS 311_ EVENN 316_ IKKKA 321_ CCCEG 326_ FPFHE 331_ VIQEF 336_ LLNKD 341_ KLVKV 346_ IRYQR 351_ PDLLL 356_ FQRFT 361_ LEKME 366_ WPNHY 371_ ACEKL 376_ LVLLT 381_ HYDMI 386_ ERKLG 391_ SRNSN 396_ QLQPI 401_ RIVKT 406_ RIRNG 411_ VHCFE 416_ IEWEK 421_ PEHYA 426_ MEDKQ 431_ HGEFA 436_ LLTIE 441_ EESLF 446_ EAAYP 451_ EIVAV 456_ YQKQK 461_ LEIKG 466_ KKQKR 471_ IKPKE 476_ NNLPE 481_ PDEVM 486_ SFQSH 491_ MTLKP 496_ TCEIF 501_ HKQNS 506_ KLNSG 511_ ISPDP 516_ TLPQE 521_ SISAS 526_ LNSLL 531_ LPKNT 536_ PCLNA 541_ QEQFM 546_ SSLRP 551_ LAIQQ 556_ IKAVS 561_ KSLIS 566_ ESSQP 571_ NTSSH 576_ NISVI 581_ ADLHL 586_ STIDW 591_ EGTSF 596_ SNSPA 601_ IQRNT 606_ FSHDL 611_ KSEVE 616_ SELSA 621_ IPDGF 626_ ENIPE 631_ QLSCE 636_ SERYT 641_ ANIKK 646_ VLDED 651_ SDGIS 656_ PEEHL 661_ LSGIT 666_ DLCLQ 671_ DLPLK 676_ ERIFT 681_ KLSYP 686_ QDNLQ 691_ PDVNL 696_ KTLSI 701_ LSVKE 706_ SCIAN 711_ SGSDC 716_ TSHLS 721_ KDLPG 726_ IPLQN 731_ ESRDS 736_ KILKG 741_ DQLLQ 746_ EDYKV 751_ NTSVP 756_ YSVSN 761_ TVVKT 766_ CNVRP 771_ PNTAL 776_ DHSRK 781_ VDMQT 786_ TRKIL 791_ MKKSV 796_ CLDRH 801_ SSDEQ 806_ SAPVF 811_ GKAKY 816_ TTQRM 821_ KHSSQ 826_ KHNSS 831_ HFKES 836_ GHNKL 841_ SSPKI 846_ HIKET 851_ EQCVR 856_ SYETA 861_ ENEES 866_ CFPDS 871_ TKSSL 876_ SSLQC 881_ HKKEN 886_ NSGTC 891_ LDSPL 896_ PLRQR 901_LKLRF
1: Endonuclease which resolves Holliday junctions (HJs) by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation (PubMed:19020614, PubMed:26682650). Cleaves HJs by a nick and counter-nick mechanism involving dual coordinated incisions that lead to the formation of ligatable nicked duplex products. Cleavage of the first strand is rate limiting, while second strand cleavage is rapid. Largely monomeric, dimerizes on the HJ and the first nick occurs upon dimerization at the junction (PubMed:26578604). Efficiently cleaves both single and double HJs contained within large recombination intermediates. Exhibits a weak sequence preference for incision between two G residues that reside in a T-rich region of DNA (PubMed:28049850). Also has endonuclease activity on 5'-flap and replication fork (RF) DNA substrates (PubMed:26578604)