GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase (EC 2.4.1.131) (Asparagine-linked glycosylation protein 11 homolog) (Glycolipid 2-alpha-mannosyltransferase)
1_MAAGE 6_ RSWCL 11_ CKLLR 16_ FFYSL 21_ FFPGL 26_ IVCGT 31_ LCVCL 36_ VIVLW 41_ GIRLL 46_ LQRKK 51_ KLVST 56_ SKNGK 61_ NQMVI 66_ AFFHP 71_ YCNAG 76_ GGGER 81_ VLWCA 86_ LRALQ 91_ KKYPE 96_ AVYVV 101_ YTGDV 106_ NVNGQ 111_ QILEG 116_ AFRRF 121_ NIRLI 126_ HPVQF 131_ VFLRK 136_ RYLVE 141_ DSLYP 146_ HFTLL 151_ GQSLG 156_ SIFLG 161_ WEALM 166_ QCVPD 171_ VYIDS 176_ MGYAF 181_ TLPLF 186_ KYIGG 191_ CQVGS 196_ YVHYP 201_ TISTD 206_ MLSVV 211_ KNQNI 216_ GFNNA 221_ AFITR 226_ NPFLS 231_ KVKLI 236_ YYYLF 241_ AFIYG 246_ LVGSC 251_ SDVVM 256_ VNSSW 261_ TLNHI 266_ LSLWK 271_ VGNCT 276_ NIVYP 281_ PCDVQ 286_ TFLDI 291_ PLHEK 296_ KMTPG 301_ HLLVS 306_ VGQFR 311_ PEKNH 316_ PLQIR 321_ AFAKL 326_ LNKKM 331_ VESPP 336_ SLKLV 341_ LIGGC 346_ RNKDD 351_ ELRVN 356_ QLRRL 361_ SEDLG 366_ VQEYV 371_ EFKIN 376_ IPFDE 381_ LKNYL 386_ SEATI 391_ GLHTM 396_ WNEHF 401_ GIGVV 406_ ECMAA 411_ GTIIL 416_ AHNSG 421_ GPKLD 426_ IVVPH 431_ EGDIT 436_ GFLAE 441_ SEEDY 446_ AETIA 451_ HILSM 456_ SAEKR 461_ LQIRK 466_ SARAS 471_ VSRFS 476_ DQEFE 481_ VTFLS 486_SVEKL
1: GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes, on the cytoplasmic face of the endoplasmic reticulum, the addition of the fourth and fifth mannose residues to the dolichol-linked oligosaccharide chain, to produce Man(5)GlcNAc(2)-PP-dolichol core oligosaccharide (PubMed:20080937). Man(5)GlcNAc(2)-PP-dolichol is a substrate for ALG3, the following enzyme in the biosynthetic pathway (PubMed:10581255)