tRNA pseudouridine synthase Pus10 (Hup10) (EC 5.4.99.25) (Coiled-coil domain-containing protein 139) (tRNA pseudouridine 55 synthase) (Psi55 synthase) (tRNA pseudouridylate synthase) (tRNA-uridine isomerase)
1_MFPLT 6_ EENKH 11_ VAQLL 16_ LNTGT 21_ CPRCI 26_ FRFCG 31_ VDFHA 36_ PYKLP 41_ YKELL 46_ NELQK 51_ FLETE 56_ KDELI 61_ LEVMN 66_ PPPKK 71_ IRLQE 76_ LEDSI 81_ DNLSQ 86_ NGEGR 91_ ISVSH 96_ VGSTA 101_ SKNSN 106_ LNVCN 111_ VCLGI 116_ LQEFC 121_ EKDFI 126_ KKVCQ 131_ KVEAS 136_ GFEFT 141_ SLVFS 146_ VSFPP 151_ QLSVR 156_ EHAAW 161_ LLVKQ 166_ EMGKQ 171_ SLSLG 176_ RDDIV 181_ QLKEA 186_ YKWIT 191_ HPLFS 196_ EELGV 201_ PIDGK 206_ SLFEV 211_ SVVFA 216_ HPETV 221_ EDCHF 226_ LAAIC 231_ PDCFK 236_ PAKNK 241_ QSVFT 246_ RMAVM 251_ KALNK 256_ IKEED 261_ FLKQF 266_ PCPPN 271_ SPKAV 276_ CAVLE 281_ IECAH 286_ GAVFV 291_ AGRYN 296_ KYSRN 301_ LPQTP 306_ WIIDG 311_ ERKLE 316_ SSVEE 321_ LISDH 326_ LLAVF 331_ KAESF 336_ NFSSS 341_ GREDV 346_ DVRTL 351_ GNGRP 356_ FAIEL 361_ VNPHR 366_ VHFTS 371_ QEIKE 376_ LQQKI 381_ NNSSN 386_ KIQVR 391_ DLQLV 396_ TREAI 401_ GHMKE 406_ GEEEK 411_ TKTYS 416_ ALIWT 421_ NKAIQ 426_ KKDIE 431_ FLNDI 436_ KDLKI 441_ DQKTP 446_ LRVLH 451_ RRPLA 456_ VRARV 461_ IHFME 466_ TQYVD 471_ EHHFR 476_ LHLKT 481_ QAGTY 486_ IKEFV 491_ HGDFG 496_ RTKPN 501_ IGSLM 506_ NVTAD 511_ ILELD 516_ VESVD 521_VDWPP
1: Protein with different functions depending on its subcellular location: involved in miRNA processing in the nucleus and acts as a tRNA pseudouridylate synthase in the cytoplasm (PubMed:31819270, PubMed:33023933). In the cytoplasm, acts as a pseudouridylate synthase by catalyzing synthesis of pseudouridine(54) and pseudouridine(55) from uracil-54 and uracil-55, respectively, in the psi GC loop of a subset of tRNAs (PubMed:30530625, PubMed:31819270, PubMed:33023933). tRNA pseudouridylate synthase activity is enhanced by the presence of 1-methyladenosine at position 53-61 of tRNAs (PubMed:30530625). Does not show tRNA pseudouridylate synthase activity in the nucleus (PubMed:33023933). In the nucleus, promotes primary microRNAs (pri-miRNAs) processing independently of its RNA pseudouridylate synthase activity (PubMed:31819270). Binds pri-miRNAs (PubMed:31819270). Modulator of TRAIL/TNFSF10-induced cell death via activation of procaspase-8 and BID cleavage (PubMed:14527409, PubMed:19712588). Required for the progression of the apoptotic signal through intrinsic mitochondrial cell death (PubMed:19712588)