Cytochrome P450 27C1 (EC 1.14.19.53) (All-trans retinol 3,4-desaturase)
1_MQTSA 6_ MALLA 11_ RILRA 16_ GLRPA 21_ PERGG 26_ LLGGG 31_ APRRP 36_ QPAGA 41_ RLPAG 46_ ARAED 51_ KGAGR 56_ PGSPP 61_ GGGRA 66_ EGPRS 71_ LAAMP 76_ GPRTL 81_ ANLAE 86_ FFCRD 91_ GFSRI 96_ HEIQQ 101_ KHTRE 106_ YGKIF 111_ KSHFG 116_ PQFVV 121_ SIADR 126_ DMVAQ 131_ VLRAE 136_ GAAPQ 141_ RANME 146_ SWREY 151_ RDLRG 156_ RATGL 161_ ISAEG 166_ EQWLK 171_ MRSVL 176_ RQRIL 181_ KPKDV 186_ AIYSG 191_ EVNQV 196_ IADLI 201_ KRIYL 206_ LRSQA 211_ EDGET 216_ VTNVN 221_ DLFFK 226_ YSMEG 231_ VATIL 236_ YESRL 241_ GCLEN 246_ SIPQL 251_ TVEYI 256_ EALEL 261_ MFSMF 266_ KTSMY 271_ AGAIP 276_ RWLRP 281_ FIPKP 286_ WREFC 291_ RSWDG 296_ LFKFS 301_ QIHVD 306_ NKLRD 311_ IQYQM 316_ DRGRR 321_ VSGGL 326_ LTYLF 331_ LSQAL 336_ TLQEI 341_ YANVT 346_ EMLLA 351_ GVDTT 356_ SFTLS 361_ WTVYL 366_ LARHP 371_ EVQQT 376_ VYREI 381_ VKNLG 386_ ERHVP 391_ TAADV 396_ PKVPL 401_ VRALL 406_ KETLR 411_ LFPVL 416_ PGNGR 421_ VTQED 426_ LVIGG 431_ YLIPK 436_ GTQLA 441_ LCHYA 446_ TSYQD 451_ ENFPR 456_ AKEFR 461_ PERWL 466_ RKGDL 471_ DRVDN 476_ FGSIP 481_ FGHGV 486_ RSCIG 491_ RRIAE 496_ LEIHL 501_ VVIQL 506_ LQHFE 511_ IKTSS 516_ QTNAV 521_ HAKTH 526_ GLLTP 531_ GGPIH 536_VRFVN
1: A cytochrome P450 monooxygenase that catalyzes the 3,4 desaturation of all-trans-retinol (also called vitamin A1) to all-trans-3,4-didehydroretinol (also called vitamin A2) in the skin. Desaturates with lower efficiency all-trans retinal and all-trans retinoic acid. Forms minor amounts of 3-hydroxy and 4-hydroxy all-trans-retinol derivatives. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin)