Dual specificity phosphatase 28 (EC 3.1.3.16) (EC 3.1.3.48)
1_MGPAE 6_ AGRRG 11_ AASPV 16_ PPPLV 21_ RVAPS 26_ LFLGS 31_ ARAAG 36_ AEEQL 41_ ARAGV 46_ TLCVN 51_ VSRQQ 56_ PGPRA 61_ PGVAE 66_ LRVPV 71_ FDDPA 76_ EDLLA 81_ HLEPT 86_ CAAME 91_ AAVRA 96_ GGACL 101_ VYCKN 106_ GRSRS 111_ AAVCT 116_ AYLMR 121_ HRGLS 126_ LAKAF 131_ QMVKS 136_ ARPVA 141_ EPNPG 146_ FWSQL 151_ QKYEE 156_ ALQAQ 161_ SCLQG 166_ EPPAL 171_GLGPE
1: Has phosphatase activity with the synthetic substrate 6,8-difluoro-4-methylumbelliferyl phosphate (in vitro) (PubMed:24531476, PubMed:29121083). Has almost no detectable activity with phosphotyrosine, even less activity with phosphothreonine and displays complete lack of activity with phosphoserine (PubMed:29121083). The poor activity with phosphotyrosine may be due to steric hindrance by bulky amino acid sidechains that obstruct access to the active site (PubMed:29121083)