Kelch-like protein 12 (CUL3-interacting protein 1) (DKIR homolog) (hDKIR)
1_MGGIM 6_ APKDI 11_ MTNTH 16_ AKSIL 21_ NSMNS 26_ LRKSN 31_ TLCDV 36_ TLRVE 41_ QKDFP 46_ AHRIV 51_ LAACS 56_ DYFCA 61_ MFTSE 66_ LSEKG 71_ KPYVD 76_ IQGLT 81_ ASTME 86_ ILLDF 91_ VYTET 96_ VHVTV 101_ ENVQE 106_ LLPAA 111_ CLLQL 116_ KGVKQ 121_ ACCEF 126_ LESQL 131_ DPSNC 136_ LGIRD 141_ FAETH 146_ NCVDL 151_ MQAAE 156_ VFSQK 161_ HFPEV 166_ VQHEE 171_ FILLS 176_ QGEVE 181_ KLIKC 186_ DEIQV 191_ DSEEP 196_ VFEAV 201_ INWVK 206_ HAKKE 211_ REESL 216_ PNLLQ 221_ YVRMP 226_ LLTPR 231_ YITDV 236_ IDAEP 241_ FIRCS 246_ LQCRD 251_ LVDEA 256_ KKFHL 261_ RPELR 266_ SQMQG 271_ PRTRA 276_ RLGAN 281_ EVLLV 286_ VGGFG 291_ SQQSP 296_ IDVVE 301_ KYDPK 306_ TQEWS 311_ FLPSI 316_ TRKRR 321_ YVASV 326_ SLHDR 331_ IYVIG 336_ GYDGR 341_ SRLSS 346_ VECLD 351_ YTADE 356_ DGVWY 361_ SVAPM 366_ NVRRG 371_ LAGAT 376_ TLGDM 381_ IYVSG 386_ GFDGS 391_ RRHTS 396_ MERYD 401_ PNIDQ 406_ WSMLG 411_ DMQTA 416_ REGAG 421_ LVVAS 426_ GVIYC 431_ LGGYD 436_ GLNIL 441_ NSVEK 446_ YDPHT 451_ GHWTN 456_ VTPMA 461_ TKRSG 466_ AGVAL 471_ LNDHI 476_ YVVGG 481_ FDGTA 486_ HLSSV 491_ EAYNI 496_ RTDSW 501_ TTVTS 506_ MTTPR 511_ CYVGA 516_ TVLRG 521_ RLYAI 526_ AGYDG 531_ NSLLS 536_ SIECY 541_ DPIID 546_ SWEVV 551_ TSMGT 556_ QRCDA 561_GVCVL
1: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a negative regulator of Wnt signaling pathway and ER-Golgi transport (PubMed:22358839, PubMed:27565346). The BCR(KLHL12) complex is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B) (PubMed:22358839, PubMed:27565346). The BCR(KLHL12) complex is also involved in neural crest specification: in response to cytosolic calcium increase, interacts with the heterodimer formed with PEF1 and PDCD6/ALG-2, leading to bridge together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export (PubMed:27716508). As part of the BCR(KLHL12) complex, also acts as a negative regulator of the Wnt signaling pathway by mediating ubiquitination and subsequent proteolysis of DVL3 (PubMed:16547521). The BCR(KLHL12) complex also mediates polyubiquitination of DRD4 and PEF1, without leading to degradation of these proteins (PubMed:18303015, PubMed:20100572, PubMed:27716508)