Single-strand selective monofunctional uracil DNA glycosylase (EC 3.2.2.-)
1_MPQAF 6_ LLGSI 11_ HEPAG 16_ ALMEP 21_ QPCPG 26_ SLAES 31_ FLEEE 36_ LRLNA 41_ ELSQL 46_ QFSEP 51_ VGIIY 56_ NPVEY 61_ AWEPH 66_ RNYVT 71_ RYCQG 76_ PKEVL 81_ FLGMN 86_ PGPFG 91_ MAQTG 96_ VPFGE 101_ VSMVR 106_ DWLGI 111_ VGPVL 116_ TPPQE 121_ HPKRP 126_ VLGLE 131_ CPQSE 136_ VSGAR 141_ FWGFF 146_ RNLCG 151_ QPEVF 156_ FHHCF 161_ VHNLC 166_ PLLFL 171_ APSGR 176_ NLTPA 181_ ELPAK 186_ QREQL 191_ LGICD 196_ AALCR 201_ QVQLL 206_ GVRLV 211_ VGVGR 216_ LAEQR 221_ ARRAL 226_ AGLMP 231_ EVQVE 236_ GLLHP 241_ SPRNP 246_ QANKG 251_ WEAVA 256_ KERLN 261_ELGLL
1: Recognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. The activity is greater toward mismatches (U/G) compared to matches (U/A). Excises uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine), nor other oxidized bases. The activity is damage-specific and salt-dependent. The substrate preference is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration, and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration