Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase (EC 2.4.1.256) (Alpha-1,2-glucosyltransferase ALG10-A) (Alpha-2-glucosyltransferase ALG10-A) (Asparagine-linked glycosylation protein 10 homolog A)
1_MAQLE 6_ GYYFS 11_ AALSC 16_ TFLVS 21_ CLLFS 26_ AFSRA 31_ LREPY 36_ MDEIF 41_ HLPQA 46_ QRYCE 51_ GHFSL 56_ SQWDP 61_ MITTL 66_ PGLYL 71_ VSIGV 76_ IKPAI 81_ WIFGW 86_ SEHVV 91_ CSIGM 96_ LRFVN 101_ LLFSV 106_ GNFYL 111_ LYLLF 116_ CKVQP 121_ RNKAA 126_ SSIQR 131_ VLSTL 136_ TLAVF 141_ PTLYF 146_ FNFLY 151_ YTEAG 156_ SMFFT 161_ LFAYL 166_ MCLYG 171_ NHKTS 176_ AFLGF 181_ CGFMF 186_ RQTNI 191_ IWAVF 196_ CAGNV 201_ IAQKL 206_ TEAWK 211_ TELQK 216_ KEDRL 221_ PPIKG 226_ PFAEF 231_ RKILQ 236_ FLLAY 241_ SMSFK 246_ NLSML 251_ LLLTW 256_ PYILL 261_ GFLFC 266_ AFVVV 271_ NGGIV 276_ IGDRS 281_ SHEAC 286_ LHFPQ 291_ LFYFF 296_ SFTLF 301_ FSFPH 306_ LLSPS 311_ KIKTF 316_ LSLVW 321_ KRRIL 326_ FFVVT 331_ LVSVF 336_ LVWKF 341_ TYAHK 346_ YLLAD 351_ NRHYT 356_ FYVWK 361_ RVFQR 366_ YETVK 371_ YLLVP 376_ AYIFA 381_ GWSIA 386_ DSLKS 391_ KSIFW 396_ NLMFF 401_ ICLFT 406_ VIVPQ 411_ KLLEF 416_ RYFIL 421_ PYVIY 426_ RLNIP 431_ LPPTS 436_ RLICE 441_ LSCYA 446_ VVNFI 451_ TFFIF 456_ LNKTF 461_ QWPNS 466_QDIQR
1: Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the third and last glucose residue from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide intermediate Glc(2)Man(9)GlcNAc(2)-PP-Dol to produce Glc(3)Man(9)GlcNAc(2)-PP-Dol