Polyserase-2 (EC 3.4.21.-) (Polyserine protease 2) (Serine protease 36)
1_MARHL 6_ LLPLV 11_ MLVIS 16_ PIPGA 21_ FQDSA 26_ LSPTQ 31_ EEPED 36_ LDCGR 41_ PEPSA 46_ RIVGG 51_ SNAQP 56_ GTWPW 61_ QVSLH 66_ HGGGH 71_ ICGGS 76_ LIAPS 81_ WVLSA 86_ AHCFM 91_ TNGTL 96_ EPAAE 101_ WSVLL 106_ GVHSQ 111_ DGPLD 116_ GAHTR 121_ AVAAI 126_ VVPAN 131_ YSQVE 136_ LGADL 141_ ALLRL 146_ ASPAS 151_ LGPAV 156_ WPVCL 161_ PRASH 166_ RFVHG 171_ TACWA 176_ TGWGD 181_ VQEAD 186_ PLPLP 191_ WVLQE 196_ VELRL 201_ LGEAT 206_ CQCLY 211_ SQPGP 216_ FNLTL 221_ QILPG 226_ MLCAG 231_ YPEGR 236_ RDTCQ 241_ GDSGG 246_ PLVCE 251_ EGGRW 256_ FQAGI 261_ TSFGF 266_ GCGRR 271_ NRPGV 276_ FTAVA 281_ TYEAW 286_ IREQV 291_ MGSEP 296_ GPAFP 301_ TQPQK 306_ TQSDP 311_ QEPRE 316_ ENCTI 321_ ALPEC 326_ GKAPR 331_ PGAWP 336_ WEAQV 341_ MVPGS 346_ RPCHG 351_ ALVSE 356_ SWVLA 361_ PASCF 366_ LDPNS 371_ SDSPP 376_ RDLDA 381_ WRVLL 386_ PSRPR 391_ AERVA 396_ RLVQH 401_ ENASW 406_ DNASD 411_ LALLQ 416_ LRTPV 421_ NLSAA 426_ SRPVC 431_ LPHPE 436_ HYFLP 441_ GSRCR 446_ LARWG 451_ RGEPA 456_ LGPGA 461_ LLEAE 466_ LLGGW 471_ WCHCL 476_ YGRQG 481_ AAVPL 486_ PGDPP 491_ HALCP 496_ AYQEK 501_ EEVGS 506_ CWNDS 511_ RWSLL 516_ CQEEG 521_ TWFLA 526_ GIRDF 531_ PSGCL 536_ RPRAF 541_ FPLQT 546_ HGPWI 551_ SHVTR 556_ GAYLE 561_ DQLAW 566_ DWGPD 571_ GEETE 576_ TQTCP 581_ PHTEH 586_ GACGL 591_ RLEAA 596_ PVGVL 601_ WPWLA 606_ EVHVA 611_ GDRVC 616_ TGILL 621_ APGWV 626_ LAATH 631_ CVLRP 636_ GSTTV 641_ PYIEV 646_ YLGRA 651_ GASSL 656_ PQGHQ 661_ VSRLV 666_ ISIRL 671_ PQHLG 676_ LRPPL 681_ ALLEL 686_ SSRVE 691_ PSPSA 696_ LPICL 701_ HPAGI 706_ PPGAS 711_ CWVLG 716_ WKEPQ 721_ DRVPV 726_ AAAVS 731_ ILTQR 736_ ICDCL 741_ YQGIL 746_ PPGTL 751_ CVLYA 756_ EGQEN 761_ RCEMT 766_ SAPPL 771_ LCQMT 776_ EGSWI 781_ LVGMA 786_ VQGSR 791_ ELFAA 796_ IGPEE 801_ AWISQ 806_ TVGEA 811_ NFLPP 816_ SGSPH 821_ WPTGG 826_ SNLCP 831_ PELAK 836_ ASGSP 841_ HAVYF 846_LLLLT
1: Serine protease. Hydrolyzes the peptides N-t-Boc-Gln-Ala-Arg-AMC and N-t-Boc-Gln-Gly-Arg-AMC and, to a lesser extent, N-t-Boc-Ala-Phe-Lys-AMC and N-t-Boc-Val-Leu-Lys-AMC. Has a preference for substrates with an Arg instead of a Lys residue in position P1