Alpha-tubulin N-acetyltransferase 1 (Alpha-TAT) (Alpha-TAT1) (TAT) (EC 2.3.1.108) (Acetyltransferase mec-17 homolog)
1_MEFPF 6_ DVDAL 11_ FPERI 16_ TVLDQ 21_ HLRPP 26_ ARRPG 31_ TTTPA 36_ RVDLQ 41_ QQIMT 46_ IIDEL 51_ GKASA 56_ KAQNL 61_ SAPIT 66_ SASRM 71_ QSNRH 76_ VVYIL 81_ KDSSA 86_ RPAGK 91_ GAIIG 96_ FIKVG 101_ YKKLF 106_ VLDDR 111_ EAHNE 116_ VEPLC 121_ ILDFY 126_ IHESV 131_ QRHGH 136_ GRELF 141_ QYMLQ 146_ KERVE 151_ PHQLA 156_ IDRPS 161_ QKLLK 166_ FLNKH 171_ YNLET 176_ TVPQV 181_ NNFVI 186_ FEGFF 191_ AHQHR 196_ PPAPS 201_ LRATR 206_ HSRAA 211_ AVDPT 216_ PAAPA 221_ RKLPP 226_ KRAEG 231_ DIKPY 236_ SSSDR 241_ EFLKV 246_ AVEPP 251_ WPLNR 256_ APRRA 261_ TPPAH 266_ PPPRS 271_ SSLGN 276_ SPERG 281_ PLRPF 286_ VPEQE 291_ LLRSL 296_ RLCPP 301_ HPTAR 306_ LLLAA 311_ DPGGS 316_ PAQRR 321_ RTRGT 326_ PPGLV 331_ AQSCC 336_ YSRHG 341_ GVNSS 346_ SPNTG 351_ NQDSK 356_ QGEQE 361_ TKNRS 366_ ASEEQ 371_ ALSQD 376_ GSGEK 381_ PMHTA 386_ PPQAP 391_ APPAQ 396_ SWTVG 401_ GDILN 406_ ARFIR 411_ NLQER 416_RSTRP
1: Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released. Required for normal sperm flagellar function. Promotes directional cell locomotion and chemotaxis, through AP2A2-dependent acetylation of alpha-tubulin at clathrin-coated pits that are concentrated at the leading edge of migrating cells. May facilitate primary cilium assembly