DDB1- and CUL4-associated factor 12 (Centrosome-related protein TCC52) (Testis cancer centrosome-related protein) (WD repeat-containing protein 40A)
1_MARKV 6_ VSRKR 11_ KAPAS 16_ PGAGS 21_ DAQGP 26_ QFGWD 31_ HSLHK 36_ RKRLP 41_ PVKRS 46_ LVYYL 51_ KNREV 56_ RLQNE 61_ TSYSR 66_ VLHGY 71_ AAQQL 76_ PSLLK 81_ EREFH 86_ LGTLN 91_ KVFAS 96_ QWLNH 101_ RQVVC 106_ GTKCN 111_ TLFVV 116_ DVQTS 121_ QITKI 126_ PILKD 131_ REPGG 136_ VTQQG 141_ CGIHA 146_ IELNP 151_ SRTLL 156_ ATGGD 161_ NPNSL 166_ AIYRL 171_ PTLDP 176_ VCVGD 181_ DGHKD 186_ WIFSI 191_ AWISD 196_ TMAVS 201_ GSRDG 206_ SMGLW 211_ EVTDD 216_ VLTKS 221_ DARHN 226_ VSRVP 231_ VYAHI 236_ THKAL 241_ KDIPK 246_ EDTNP 251_ DNCKV 256_ RALAF 261_ NNKNK 266_ ELGAV 271_ SLDGY 276_ FHLWK 281_ AENTL 286_ SKLLS 291_ TKLPY 296_ CRENV 301_ CLAYG 306_ SEWSV 311_ YAVGS 316_ QAHVS 321_ FLDPR 326_ QPSYN 331_ VKSVC 336_ SRERG 341_ SGIRS 346_ VSFYE 351_ HIITV 356_ GTGQG 361_ SLLFY 366_ DIRAQ 371_ RFLEE 376_ RLSAC 381_ YGSKP 386_ RLAGE 391_ NLKLT 396_ TGKGW 401_ LNHDE 406_ TWRNY 411_ FSDID 416_ FFPNA 421_ VYTHC 426_ YDSSG 431_ TKLFV 436_ AGGPL 441_ PSGLH 446_GNYAG
1: Substrate-recognition component of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation (PubMed:16949367, PubMed:16964240, PubMed:29779948). The C-degron recognized by the DesCEND pathway is usually a motif of less than ten residues and can be present in full-length proteins, truncated proteins or proteolytically cleaved forms (PubMed:29779948). The DCX(DCAF12) complex specifically recognizes proteins with a diglutamate (Glu-Glu) at the C-terminus, such as MAGEA3, MAGEA6 and CCT5, leading to their ubiquitination and degradation (PubMed:29779948, PubMed:31267705). Ubiquitination of MAGEA3, MAGEA6 by DCX(DCAF12) complex is required for starvation-induced autophagy (PubMed:31267705). Also directly recognizes the C-terminal glutamate-leucine (Glu-Leu) degron as an alternative degron in proteins such as MOV10, leading to their ubiquitination and degradation. Controls the protein level of MOV10 during spermatogenesis and in T cells, especially after their activation (PubMed:34065512)