Queuosine salvage protein
1_MDGLL 6_ NPRES 11_ SKFIA 16_ ENSRD 21_ VFIDS 26_ GGVRR 31_ VAELL 36_ LAKAA 41_ GPELR 46_ VEGWK 51_ ALHEL 56_ NPRAA 61_ DEAAV 66_ NWVFV 71_ TDTLN 76_ FSFWS 81_ EQDEH 86_ KCVVR 91_ YRGKT 96_ YSGYW 101_ SLCAA 106_ VNRAL 111_ DEGIP 116_ ITSAS 121_ YYATV 126_ TLDQV 131_ RNILR 136_ SDTDV 141_ SMPLV 146_ EERHR 151_ ILNET 156_ GKILL 161_ EKFGG 166_ SFLNC 171_ VRESE 176_ NSAQK 181_ LMHLV 186_ VESFP 191_ SYRDV 196_ TLFEG 201_ KRVSF 206_ YKRAQ 211_ ILVAD 216_ TWSVL 221_ EGKGD 226_ GCFKD 231_ ISSIT 236_ MFADY 241_ RLPQV 246_ LAHLG 251_ ALKYS 256_ DDLLK 261_ KLLKG 266_ EMLSY 271_ GDRQE 276_ VEIRG 281_ CSLWC 286_ VELIR 291_ DCLLE 296_ LIEQK 301_ GEKPN 306_ GEINS 311_ ILLDY 316_ YLWDY 321_ AHDHR 326_ EDMKG 331_ IPFHR 336_IRCIY
1: Catalyzes the hydrolysis of queuosine 5'-phosphate, releasing the nucleobase queuine (q). Is required for salvage of queuine from exogenous queuosine (Q) that is imported and then converted to queuosine 5'-phosphate intracellularly. In vitro, can also catalyze the release of the q base directly from Q as substrate; however, it was shown that Q is not the biologically relevant substrate. Shows a very low activity on queuosine 3',5'-diphosphate, and cannot release q from queuosine 3'-phosphate and from the 5'-nucleotides AMP, UMP, CMP or GMP, indicating specificity for the queuine base (PubMed:36610787). Can complement the yeast mutant SPAC589.05c, restoring Q incorporation into tRNA (PubMed:24911101)