Terminal uridylyltransferase 7 (TUTase 7) (EC 2.7.7.52) (Zinc finger CCHC domain-containing protein 6)
1_MGDTA 6_ KPYFV 11_ KRTKD 16_ RGTMD 21_ DDDFR 26_ RGHPQ 31_ QDYLI 36_ IDDHA 41_ KGHGS 46_ KMEKG 51_ LQKKK 56_ ITPGN 61_ YGNTP 66_ RKGPC 71_ AVSSN 76_ PYAFK 81_ NPIYS 86_ QPAWM 91_ NDSHK 96_ DQSKR 101_ WLSDE 106_ HTGNS 111_ DNWRE 116_ FKPGP 121_ RIPVI 126_ NRQRK 131_ DSFQE 136_ NEDGY 141_ RWQDT 146_ RGCRT 151_ VRRLF 156_ HKDLT 161_ SLETT 166_ SEMEA 171_ GSPEN 176_ KKQRS 181_ RPRKP 186_ RKTRN 191_ EENEQ 196_ DGDLE 201_ GPVID 206_ ESVLS 211_ TKELL 216_ GLQQA 221_ EERLK 226_ RDCID 231_ RLKRR 236_ PRNYP 241_ TAKYT 246_ CRLCD 251_ VLIES 256_ IAFAH 261_ KHIKE 266_ KRHKK 271_ NIKEK 276_ QEEEL 281_ LTTLP 286_ PPTPS 291_ QINAV 296_ GIAID 301_ KVVQE 306_ FGLHN 311_ ENLEQ 316_ RLEIK 321_ RIMEN 326_ VFQHK 331_ LPDCS 336_ LRLYG 341_ SSCSR 346_ LGFKN 351_ SDVNI 356_ DIQFP 361_ AIMSQ 366_ PDVLL 371_ LVQEC 376_ LKNSD 381_ SFIDV 386_ DADFH 391_ ARVPV 396_ VVCRE 401_ KQSGL 406_ LCKVS 411_ AGNEN 416_ ACLTT 421_ KHLTA 426_ LGKLE 431_ PKLVP 436_ LVIAF 441_ RYWAK 446_ LCSID 451_ RPEEG 456_ GLPPY 461_ VFALM 466_ AIFFL 471_ QQRKE 476_ PLLPV 481_ YLGSW 486_ IEGFS 491_ LSKLG 496_ NFNLQ 501_ DIEKD 506_ VVIWE 511_ HTDSA 516_ AGDTG 521_ ITKEE 526_ APRET 531_ PIKRG 536_ QVSLI 541_ LDVKH 546_ QPSVP 551_ VGQLW 556_ VELLR 561_ FYALE 566_ FNLAD 571_ LVISI 576_ RVKEL 581_ VSREL 586_ KDWPK 591_ KRIAI 596_ EDPYS 601_ VKRNV 606_ ARTLN 611_ SQPVF 616_ EYILH 621_ CLRTT 626_ YKYFA 631_ LPHKI 636_ TKSSL 641_ LKPLN 646_ AITCI 651_ SEHSK 656_ EVINH 661_ HPDVQ 666_ TKDDK 671_ LKNSV 676_ LAQGP 681_ GATSS 686_ AANTC 691_ KVQPL 696_ TLKET 701_ AESFG 706_ SPPKE 711_ EMGNE 716_ HISVH 721_ PENSD 726_ CIQAD 731_ VNSDD 736_ YKGDK 741_ VYHPE 746_ TGRKN 751_ EKEKV 756_ GRKGK 761_ HLLTV 766_ DQKRG 771_ EHVVC 776_ GSTRN 781_ NESES 786_ TLDLE 791_ GFQNP 796_ TAKEC 801_ EGLAT 806_ LDNKA 811_ DLDGE 816_ STEGT 821_ EELED 826_ SLNHF 831_ THSVQ 836_ GQTSE 841_ MIPSD 846_ EEEED 851_ DEEEE 856_ EEEEP 861_ RLTIN 866_ QREDE 871_ DGMAN 876_ EDELD 881_ NTYTG 886_ SGDED 891_ ALSEE 896_ DDELG 901_ EAAKY 906_ EDVKE 911_ CGKHV 916_ ERALL 921_ VELNK 926_ ISLKE 931_ ENVCE 936_ EKNSP 941_ VDQSD 946_ FFYEF 951_ SKLIF 956_ TKGKS 961_ PTVVC 966_ SLCKR 971_ EGHLK 976_ KDCPE 981_ DFKRI 986_ QLEPL 991_ PPLTP 996_ KFLNI 1001_ LDQVC 1006_ IQCYK 1011_ DFSPT 1016_ IIEDQ 1021_ AREHI 1026_ RQNLE 1031_ SFIRQ 1036_ DFPGT 1041_ KLSLF 1046_ GSSKN 1051_ GFGFK 1056_ QSDLD 1061_ VCMTI 1066_ NGLET 1071_ AEGLD 1076_ CVRTI 1081_ EELAR 1086_ VLRKH 1091_ SGLRN 1096_ ILPIT 1101_ TAKVP 1106_ IVKFF 1111_ HLRSG 1116_ LEVDI 1121_ SLYNT 1126_ LALHN 1131_ TRLLS 1136_ AYSAI 1141_ DPRVK 1146_ YLCYT 1151_ MKVFT 1156_ KMCDI 1161_ GDASR 1166_ GSLSS 1171_ YAYTL 1176_ MVLYF 1181_ LQQRN 1186_ PPVIP 1191_ VLQEI 1196_ YKGEK 1201_ KPEIF 1206_ VDGWN 1211_ IYFFD 1216_ QIDEL 1221_ PTYWS 1226_ ECGKN 1231_ TESVG 1236_ QLWLG 1241_ LLRFY 1246_ TEEFD 1251_ FKEHV 1256_ ISIRR 1261_ KSLLT 1266_ TFKKQ 1271_ WTSKY 1276_ IVIED 1281_ PFDLN 1286_ HNLGA 1291_ GLSRK 1296_ MTNFI 1301_ MKAFI 1306_ NGRRV 1311_ FGIPV 1316_ KGFPK 1321_ DYPSK 1326_ MEYFF 1331_ DPDVL 1336_ TEGEL 1341_ APNDR 1346_ CCRIC 1351_ GKIGH 1356_ FMKDC 1361_ PMRRK 1366_ VRRRR 1371_ DQEDA 1376_ LNQRY 1381_ PENKE 1386_ KRSKE 1391_ DKEIH 1396_ NKYTE 1401_ REVST 1406_ KEDKP 1411_ IQCTP 1416_ QKAKP 1421_ MRAAA 1426_ DLGRE 1431_ KILRP 1436_ PVEKW 1441_ KRQDD 1446_ KDLRE 1451_ KRCFI 1456_ CGREG 1461_ HIKKE 1466_ CPQFK 1471_ GSSGS 1476_ LSSKY 1481_ MTQGK 1486_ASAKR
1: Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay (PubMed:19703396, PubMed:25480299). Essential for both oocyte maturation and fertility. Through 3' terminal uridylation of mRNA, sculpts, with TUT7, the maternal transcriptome by eliminating transcripts during oocyte growth (By similarity). Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets (PubMed:25480299). Also functions as an integral regulator of microRNA biogenesiS using 3 different uridylation mechanisms (PubMed:25979828). Acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7). Uridylated pre-let-7 RNA is not processed by Dicer and undergo degradation. Pre-let-7 uridylation is strongly enhanced in the presence of LIN28A (PubMed:22898984). In the absence of LIN28A, TUT7 and TUT4 monouridylate group II pre-miRNAs, which includes most of pre-let7 members, that shapes an optimal 3' end overhang for efficient processing (PubMed:25979828, PubMed:28671666). Add oligo-U tails to truncated pre-miRNAS with a 5' overhang which may promote rapid degradation of non-functional pre-miRNA species (PubMed:25979828). Does not play a role in replication-dependent histone mRNA degradation (PubMed:18172165). Due to functional redundancy between TUT4 and TUT7, the identification of the specific role of each of these proteins is difficult (PubMed:18172165, PubMed:19703396, PubMed:22898984, PubMed:25480299, PubMed:25979828, PubMed:28671666). TUT4 and TUT7 restrict retrotransposition of long interspersed element-1 (LINE-1) in cooperation with MOV10 counteracting the RNA chaperonne activity of L1RE1. TUT7 uridylates LINE-1 mRNAs in the cytoplasm which inhibits initiation of reverse transcription once in the nucleus, whereas uridylation by TUT4 destabilizes mRNAs in cytoplasmic ribonucleoprotein granules (PubMed:30122351)