Ultra-long-chain fatty acid omega-hydroxylase (EC 1.14.14.177) (Cytochrome P450 4F22)
1_MLPIT 6_ DRLLH 11_ LLGLE 16_ KTAFR 21_ IYAVS 26_ TLLLF 31_ LLFFL 36_ FRLLL 41_ RFLRL 46_ CRSFY 51_ ITCRR 56_ LRCFP 61_ QPPRR 66_ NWLLG 71_ HLGMY 76_ LPNEA 81_ GLQDE 86_ KKVLD 91_ NMHHV 96_ LLVWM 101_ GPVLP 106_ LLVLV 111_ HPDYI 116_ KPLLG 121_ ASAAI 126_ APKDD 131_ LFYGF 136_ LKPWL 141_ GDGLL 146_ LSKGD 151_ KWSRH 156_ RRLLT 161_ PAFHF 166_ DILKP 171_ YMKIF 176_ NQSAD 181_ IMHAK 186_ WRHLA 191_ EGSAV 196_ SLDMF 201_ EHISL 206_ MTLDS 211_ LQKCV 216_ FSYNS 221_ NCQEK 226_ MSDYI 231_ SAIIE 236_ LSALS 241_ VRRQY 246_ RLHHY 251_ LDFIY 256_ YRSAD 261_ GRRFR 266_ QACDM 271_ VHHFT 276_ TEVIQ 281_ ERRRA 286_ LRQQG 291_ AEAWL 296_ KAKQG 301_ KTLDF 306_ IDVLL 311_ LARDE 316_ DGKEL 321_ SDEDI 326_ RAEAD 331_ TFMFE 336_ GHDTT 341_ SSGIS 346_ WMLFN 351_ LAKYP 356_ EYQEK 361_ CREEI 366_ QEVMK 371_ GRELE 376_ ELEWD 381_ DLTQL 386_ PFTTM 391_ CIKES 396_ LRQYP 401_ PVTLV 406_ SRQCT 411_ EDIKL 416_ PDGRI 421_ IPKGI 426_ ICLVS 431_ IYGTH 436_ HNPTV 441_ WPDSK 446_ VYNPY 451_ RFDPD 456_ NPQQR 461_ SPLAY 466_ VPFSA 471_ GPRNC 476_ IGQSF 481_ AMAEL 486_ RVVVA 491_ LTLLR 496_ FRLSV 501_ DRTRK 506_ VRRKP 511_ ELILR 516_ TENGL 521_ WLKVE 526_PLPPR
1: A cytochrome P450 monooxygenase involved in epidermal ceramide biosynthesis. Hydroxylates the terminal carbon (omega-hydroxylation) of ultra-long-chain fatty acyls (C28-C36) prior to ceramide synthesis (PubMed:26056268). Contributes to the synthesis of three classes of omega-hydroxy-ultra-long chain fatty acylceramides having sphingosine, 6-hydroxysphingosine and phytosphingosine bases, all major lipid components that underlie the permeability barrier of the stratum corneum (PubMed:26056268). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:26056268)