S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1B (EC 4.1.3.44) (Radical S-adenosyl methionine and flavodoxin domain-containing protein 2) (tRNA wybutosine-synthesizing protein 1 homolog B)
1_MDPSA 6_ DTWDL 11_ SSPLI 16_ SLWIN 21_ RFYIY 26_ LGFAV 31_ SISLW 36_ ICVQI 41_ VIEMQ 46_ GFATV 51_ LAEAV 56_ TSLDL 61_ PVAII 66_ NLKEY 71_ DPDDH 76_ LIEEV 81_ TSKNV 86_ CVFLV 91_ ATYTD 96_ GLPTE 101_ SAEWF 106_ CKWLE 111_ EASID 116_ FRFGK 121_ TYLKG 126_ MRDAV 131_ FGLGN 136_ SAYAS 141_ HFNKV 146_ GKNVD 151_ KWLWM 156_ LGVHR 161_ VMSRG 166_ EGDCD 171_ VVKSK 176_ HGSIE 181_ ANFRA 186_ WKTKF 191_ ISQLQ 196_ ALQKG 201_ ERKKS 206_ CGGHC 211_ KKGKC 216_ ESHQH 221_ GSEER 226_ EEGSQ 231_ EQDEL 236_ HHRDT 241_ KEEEP 246_ FESSS 251_ EEEFG 256_ GEDHQ 261_ SLNSI 266_ VDVED 271_ LGKIM 276_ DHVKK 281_ EKREK 286_ EQQEE 291_ KSGLF 296_ RNMGR 301_ NEDGE 306_ RRAMI 311_ TPALR 316_ EALTK 321_ QVDAP 326_ RERSL 331_ LQTHI 336_ LWNES 341_ HRCME 346_ TTPSL 351_ ACANK 356_ CVFCW 361_ WHHNN 366_ PVGTE 371_ WLWKM 376_ DQPEM 381_ ILKEA 386_ IENHQ 391_ NMIKQ 396_ FKGVP 401_ GVKAE 406_ RFEEG 411_ MTVKH 416_ CALSL 421_ VGEPI 426_ MYPEI 431_ NRFLK 436_ LLHQC 441_ KISSF 446_ LVTNA 451_ QFPAE 456_ IRNLE 461_ PVTQL 466_ YVSVD 471_ ASTKD 476_ SLKKI 481_ DRPLF 486_ KDFWQ 491_ QFLDS 496_ LKALA 501_ VKQQR 506_ TVYRL 511_ MLVKA 516_ WNVDE 521_ LQAYA 526_ QLVSL 531_ GNPDF 536_ IEVKG 541_ VTYCR 546_ ESSAS 551_ SLTMA 556_ HVPWH 561_ EEVVQ 566_ FVREL 571_ VDLIP 576_ EYEIA 581_ CEHEH 586_ SNCLL 591_ IAHRK 596_ FKIGG 601_ EWWTW 606_ IDYNR 611_ FQELI 616_ QEYED 621_ SGGSK 626_ TFSAK 631_ DYMAR 636_ TPHWA 641_ LFGAN 646_ ERSFD 651_ PKDTR 656_ HQRKN 661_KSKAI
1: Probable component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine, an intermediate in wybutosine biosynthesis (By similarity)