RNA demethylase ALKBH5 (EC 1.14.11.53) (Alkylated DNA repair protein alkB homolog 5) (Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5)
1_MAAAS 6_ GYTDL 11_ REKLK 16_ SMTSR 21_ DNYKA 26_ GSREA 31_ AAAAA 36_ AAVAA 41_ AAAAA 46_ AAAEP 51_ YPVSG 56_ AKRKY 61_ QEDSD 66_ PERSD 71_ YEEQQ 76_ LQKEE 81_ EARKV 86_ KSGIR 91_ QMRLF 96_ SQDEC 101_ AKIEA 106_ RIDEV 111_ VSRAE 116_ KGLYN 121_ EHTVD 126_ RAPLR 131_ NKYFF 136_ GEGYT 141_ YGAQL 146_ QKRGP 151_ GQERL 156_ YPPGD 161_ VDEIP 166_ EWVHQ 171_ LVIQK 176_ LVEHR 181_ VIPEG 186_ FVNSA 191_ VINDY 196_ QPGGC 201_ IVSHV 206_ DPIHI 211_ FERPI 216_ VSVSF 221_ FSDSA 226_ LCFGC 231_ KFQFK 236_ PIRVS 241_ EPVLS 246_ LPVRR 251_ GSVTV 256_ LSGYA 261_ ADEIT 266_ HCIRP 271_ QDIKE 276_ RRAVI 281_ ILRKT 286_ RLDAP 291_ RLETK 296_ SLSSS 301_ VLPPS 306_ YASDR 311_ LSGNN 316_ RDPAL 321_ KPKRS 326_ HRKAD 331_ PDAAH 336_ RPRIL 341_ EMDKE 346_ ENRRS 351_ VLLPT 356_ HRRRG 361_ SFSSE 366_ NYWRK 371_ SYESS 376_ EDCSE 381_ AAGSP 386_ARKVK
1: Dioxygenase that specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:23177736, PubMed:24489119, PubMed:24616105, PubMed:24778178, PubMed:34048572, PubMed:36944332, PubMed:37257451, PubMed:37369679). Demethylates RNA by oxidative demethylation, which requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:21264265, PubMed:23177736, PubMed:24489119, PubMed:24616105, PubMed:24778178). Demethylation of m6A mRNA affects mRNA processing, translation and export (PubMed:23177736, PubMed:34048572, PubMed:36944332, PubMed:37257451). Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro) (PubMed:24616105). Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3'-UTR mRNAs in male germ cells (By similarity). Involved in paraspeckle assembly, a nuclear membraneless organelle, by undergoing liquid-liquid phase separation (PubMed:37369679, PubMed:37474102). Paraspeckle assembly is coupled with m6A demethylation of RNAs, such as NEAT1 non-coding RNA (PubMed:37474102). Also acts as a negative regulator of T-cell development: inhibits gamma-delta T-cell proliferation via demethylation of JAG1 and NOTCH2 transcripts (By similarity). Inhibits regulatory T-cell (Treg) recruitment by mediating demethylation and destabilization of CCL28 mRNAs (By similarity)