SPRY domain-containing SOCS box protein 3 (SSB-3)
1_MARRP 6_ RNSRA 11_ WHFVL 16_ SAARR 21_ DADAR 26_ AVALA 31_ GSTNW 36_ GYDSD 41_ GQHSD 46_ SDSDP 51_ EYSTL 56_ PPSIP 61_ SAVPV 66_ TGESF 71_ CDCAG 76_ QSEAS 81_ FCSSL 86_ HSAHR 91_ GRDCR 96_ CGEED 101_ EYFDW 106_ VWDDL 111_ NKSSA 116_ TLLSC 121_ DNRKV 126_ SFHME 131_ YSCGT 136_ AAIRG 141_ TKELG 146_ EGQHF 151_ WEIKM 156_ TSPVY 161_ GTDMM 166_ VGIGT 171_ SDVDL 176_ DKYRH 181_ TFCSL 186_ LGRDE 191_ DSWGL 196_ SYTGL 201_ LHHKG 206_ DKTSF 211_ SSRFG 216_ QGSII 221_ GVHLD 226_ TWHGT 231_ LTFFK 236_ NRKCI 241_ GVAAT 246_ KLQNK 251_ RFYPM 256_ VCSTA 261_ ARSSM 266_ KVTRS 271_ CASAT 276_ SLQYL 281_ CCHRL 286_ RQLRP 291_ DSGDT 296_ LEGLP 301_ LPPGL 306_ KQVLH 311_ NKLGW 316_ VLSMS 321_ CSRRK 326_ APVSD 331_ PQAAT 336_ SAHPS 341_ SREPR 346_PCQRK
1: Substrate-recognition component of a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex, also named CRL5 complex), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as CGAS and SNAI1 (PubMed:29059170, PubMed:38418882). The ECS(SPSB3) complex catalyzes 'Lys-48'-linked ubiquitination of nuclear CGAS in cycling cells, leading to its degradation (PubMed:38418882). Recognizes and binds nucleosome-bound CGAS: ubiquitination and degradation of nuclear CGAS during G1 and G2 phases is required to promote low intranuclear CGAS abundance before the next mitotic cycle (PubMed:38418882). The ECS(SPSB3) complex also mediates ubiquitination and degradation of phosphorylated SNAI1 (PubMed:29059170)