Vitamin D 25-hydroxylase (EC 1.14.14.24) (Cytochrome P450 2R1)
1_MWKLW 6_ RAEEG 11_ AAALG 16_ GALFL 21_ LLFAL 26_ GVRQL 31_ LKQRR 36_ PMGFP 41_ PGPPG 46_ LPFIG 51_ NIYSL 56_ AASSE 61_ LPHVY 66_ MRKQS 71_ QVYGE 76_ IFSLD 81_ LGGIS 86_ TVVLN 91_ GYDVV 96_ KECLV 101_ HQSEI 106_ FADRP 111_ CLPLF 116_ MKMTK 121_ MGGLL 126_ NSRYG 131_ RGWVD 136_ HRRLA 141_ VNSFR 146_ YFGYG 151_ QKSFE 156_ SKILE 161_ ETKFF 166_ NDAIE 171_ TYKGR 176_ PFDFK 181_ QLITN 186_ AVSNI 191_ TNLII 196_ FGERF 201_ TYEDT 206_ DFQHM 211_ IELFS 216_ ENVEL 221_ AASAS 226_ VFLYN 231_ AFPWI 236_ GILPF 241_ GKHQQ 246_ LFRNA 251_ AVVYD 256_ FLSRL 261_ IEKAS 266_ VNRKP 271_ QLPQH 276_ FVDAY 281_ LDEMD 286_ QGKND 291_ PSSTF 296_ SKENL 301_ IFSVG 306_ ELIIA 311_ GTETT 316_ TNVLR 321_ WAILF 326_ MALYP 331_ NIQGQ 336_ VQKEI 341_ DLIMG 346_ PNGKP 351_ SWDDK 356_ CKMPY 361_ TEAVL 366_ HEVLR 371_ FCNIV 376_ PLGIF 381_ HATSE 386_ DAVVR 391_ GYSIP 396_ KGTTV 401_ ITNLY 406_ SVHFD 411_ EKYWR 416_ DPEVF 421_ HPERF 426_ LDSSG 431_ YFAKK 436_ EALVP 441_ FSLGR 446_ RHCLG 451_ EHLAR 456_ MEMFL 461_ FFTAL 466_ LQRFH 471_ LHFPH 476_ ELVPD 481_ LKPRL 486_ GMTLQ 491_ PQPYL 496_ICAER
1: A cytochrome P450 monooxygenase involved in activation of vitamin D precursors. Catalyzes hydroxylation at C-25 of both forms of vitamin D, vitamin D(2) and D(3) (calciol) (PubMed:12867411, PubMed:15465040, PubMed:18511070). Can metabolize vitamin D analogs/prodrugs 1alpha-hydroxyvitamin D(2) (doxercalciferol) and 1alpha-hydroxyvitamin D(3) (alfacalcidol) forming 25-hydroxy derivatives (PubMed:15465040, PubMed:18511070). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:12867411, PubMed:15465040, PubMed:18511070)