Cytoplasmic tRNA 2-thiolation protein 1 (EC 2.7.7.-) (ATP-binding domain-containing protein 3) (Cancer-associated gene protein) (Cytoplasmic tRNA adenylyltransferase 1)
1_MPAPP 6_ CASCH 11_ AARAA 16_ LRRPL 21_ SGQAL 26_ CGACF 31_ CAAFE 36_ AEVLH 41_ TVLAG 46_ RLLPP 51_ GAVVA 56_ VGASG 61_ GKDST 66_ VLAHV 71_ LRALA 76_ PRLGI 81_ SLQLV 86_ AVDEG 91_ IGGYR 96_ DAALA 101_ AVRRQ 106_ AARWE 111_ LPLTV 116_ VAYED 121_ LFGGW 126_ TMDAV 131_ ARSTA 136_ GSGRS 141_ RSCCT 146_ FCGVL 151_ RRRAL 156_ EEGAR 161_ RVGAT 166_ HIVTG 171_ HNADD 176_ MAETV 181_ LMNFL 186_ RGDAG 191_ RLARG 196_ GGLGS 201_ PGEGG 206_ ALPRC 211_ RPLQF 216_ ASQKE 221_ VVLYA 226_ HFRRL 231_ DYFSE 236_ ECVYA 241_ PEAFR 246_ GHARD 251_ LLKRL 256_ EAARP 261_ SAVLD 266_ LVHSA 271_ ERLAL 276_ APAAR 281_ PPRPG 286_ ACSRC 291_ GALAS 296_ RALCQ 301_ ACALL 306_ DGLNR 311_ GRPRL 316_ AIGKG 321_ RRGLD 326_ EEATP 331_ GTPGD 336_ PARPP 341_ASKAV
1: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position