E3 ubiquitin-protein ligase ZNF598 (EC 2.3.2.27) (Zinc finger protein 598)
1_MAAAG 6_ GAEGR 11_ RAALE 16_ AAAAA 21_ APERG 26_ GGSCV 31_ LCCGD 36_ LEATA 41_ LGRCD 46_ HPVCY 51_ RCSTK 56_ MRVLC 61_ EQRYC 66_ AVCRE 71_ ELRQV 76_ VFGKK 81_ LPAFA 86_ TIPIH 91_ QLQHE 96_ KKYDI 101_ YFADG 106_ KVYAL 111_ YRQLL 116_ QHECP 121_ RCPEL 126_ PPFSL 131_ FGDLE 136_ QHMRR 141_ QHELF 146_ CCRLC 151_ LQHLQ 156_ IFTYE 161_ RKWYS 166_ RKDLA 171_ RHRMQ 176_ GDPDD 181_ TSHRG 186_ HPLCK 191_ FCDER 196_ YLDND 201_ ELLKH 206_ LRRDH 211_ YFCHF 216_ CDSDG 221_ AQDYY 226_ SDYAY 231_ LREHF 236_ REKHF 241_ LCEEG 246_ RCSTE 251_ QFTHA 256_ FRTEI 261_ DLKAH 266_ RTACH 271_ SRSRA 276_ EARQN 281_ RHIDL 286_ QFSYA 291_ PRHSR 296_ RNEGV 301_ VGGED 306_ YEEVD 311_ RYSRQ 316_ GRVAR 321_ AGTRG 326_ AQQSR 331_ RGSWR 336_ YKREE 341_ EDREV 346_ AAAVR 351_ ASVAA 356_ QQQEE 361_ ARRSE 366_ DQEEG 371_ GRPKK 376_ EEAAA 381_ RGPED 386_ PRGPR 391_ RSPRT 396_ QGEGP 401_ GPKET 406_ STNGP 411_ VSQEA 416_ FSVTG 421_ PAAPG 426_ CVGVP 431_ GALPP 436_ PSPKL 441_ KDEDF 446_ PSLSA 451_ STSSS 456_ CSTAA 461_ TPGPV 466_ GLALP 471_ YAIPA 476_ RGRSA 481_ FQEED 486_ FPALV 491_ SSVPK 496_ PGTAP 501_ TSLVS 506_ AWNSS 511_ SSSKK 516_ VAQPP 521_ LSAQA 526_ TGSGQ 531_ PTRKA 536_ GKGSR 541_ GGRKG 546_ GPPFT 551_ QEEEE 556_ DGGPA 561_ LQELL 566_ STRPT 571_ GSVSS 576_ TLGLA 581_ SIQPS 586_ KVGKK 591_ KKVGS 596_ EKPGT 601_ TLPQP 606_ PPATC 611_ PPGAL 616_ QAPEA 621_ PASRA 626_ EGPVA 631_ VVVNG 636_ HTEGP 641_ APARS 646_ APKEP 651_ PGLPR 656_ PLGSF 661_ PCPTP 666_ QEDFP 671_ ALGGP 676_ CPPRM 681_ PPPPG 686_ FSAVV 691_ LLKGT 696_ PPPPP 701_ PGLVP 706_ PISKP 711_ PPGFS 716_ GLLPS 721_ PHPAC 726_ VPSPA 731_ TTTTT 736_ KAPRL 741_ LPAPR 746_ AYLVP 751_ ENFRE 756_ RNLQL 761_ IQSIR 766_ DFLQS 771_ DEARF 776_ SEFKS 781_ HSGEF 786_ RQGLI 791_ SAAQY 796_ YKSCR 801_ DLLGE 806_ NFQKV 811_ FNELL 816_ VLLPD 821_ TAKQQ 826_ ELLSA 831_ HTDFC 836_ NREKP 841_ LSTKS 846_ KKNKK 851_ SAWQA 856_ TTQQA 861_ GLDCR 866_ VCPTC 871_ QQVLA 876_ HGDAS 881_ SHQAL 886_ HAARD 891_ DDFPS 896_LQAIA
1: E3 ubiquitin-protein ligase that plays a key role in the ribosome quality control (RQC), a pathway that takes place when a ribosome has stalled during translation, leading to degradation of nascent peptide chains (PubMed:28065601, PubMed:28132843, PubMed:28685749, PubMed:32099016, PubMed:32579943, PubMed:33581075). ZNF598 is activated when ribosomes are stalled within an mRNA following translation of prematurely polyadenylated mRNAs (PubMed:28065601, PubMed:28132843, PubMed:28685749). Acts as a ribosome collision sensor: specifically recognizes and binds collided di-ribosome, which arises when a trailing ribosome encounters a slower leading ribosome, leading to terminally arrest translation (PubMed:28065601, PubMed:28132843, PubMed:28685749, PubMed:30293783). Following binding to colliding ribosomes, mediates monoubiquitination of 40S ribosomal proteins RPS10/eS10 and RPS3/uS3, and 'Lys-63'-linked polyubiquitination of RPS20/uS10 (PubMed:28065601, PubMed:28132843, PubMed:28685749). Polyubiquitination of RPS20/uS10 promotes recruitment of the RQT (ribosome quality control trigger) complex, which drives the disassembly of stalled ribosomes, followed by degradation of nascent peptides (PubMed:32099016, PubMed:32579943, PubMed:36302773). E3 ubiquitin-protein ligase activity is dependent on the E2 ubiquitin-conjugating enzyme UBE2D3 (PubMed:28685749). Also acts as an adapter that recruits the 4EHP-GYF2 complex to mRNAs (PubMed:22751931, PubMed:32726578). Independently of its role in RQC, may also act as a negative regulator of interferon-stimulated gene (ISG) expression (PubMed:29719242)
2: (Microbial infection) Required for poxvirus protein synthesis by mediating ubiquitination of RPS10/eS10 and RPS20/uS10 (PubMed:29719242). Poxvirus encoding mRNAs contain unusual 5' poly(A) leaders and ZNF598 is required for their translational efficiency, possibly via its ability to suppress readthrough or sliding on shorter poly(A) tracts (PubMed:29719242)