Cytochrome P450 4Z1 (EC 1.14.14.1) (CYPIVZ1) (Laurate 7-monooxygenase) (EC 1.14.14.130)
1_MEPSW 6_ LQELM 11_ AHPFL 16_ LLILL 21_ CMSLL 26_ LFQVI 31_ RLYQR 36_ RRWMI 41_ RALHL 46_ FPAPP 51_ AHWFY 56_ GHKEF 61_ YPVKE 66_ FEVYH 71_ KLMEK 76_ YPCAV 81_ PLWVG 86_ PFTMF 91_ FSVHD 96_ PDYAK 101_ ILLKR 106_ QDPKS 111_ AVSHK 116_ ILESW 121_ VGRGL 126_ VTLDG 131_ SKWKK 136_ HRQIV 141_ KPGFN 146_ ISILK 151_ IFITM 156_ MSESV 161_ RMMLN 166_ KWEEH 171_ IAQNS 176_ RLELF 181_ QHVSL 186_ MTLDS 191_ IMKCA 196_ FSHQG 201_ SIQLD 206_ STLDS 211_ YLKAV 216_ FNLSK 221_ ISNQR 226_ MNNFL 231_ HHNDL 236_ VFKFS 241_ SQGQI 246_ FSKFN 251_ QELHQ 256_ FTEKV 261_ IQDRK 266_ ESLKD 271_ KLKQD 276_ TTQKR 281_ RWDFL 286_ DILLS 291_ AKSEN 296_ TKDFS 301_ EADLQ 306_ AEVKT 311_ FMFAG 316_ HDTTS 321_ SAISW 326_ ILYCL 331_ AKYPE 336_ HQQRC 341_ RDEIR 346_ ELLGD 351_ GSSIT 356_ WEHLS 361_ QMPYT 366_ TMCIK 371_ ECLRL 376_ YAPVV 381_ NISRL 386_ LDKPI 391_ TFPDG 396_ RSLPA 401_ GITVF 406_ INIWA 411_ LHHNP 416_ YFWED 421_ PQVFN 426_ PLRFS 431_ RENSE 436_ KIHPY 441_ AFIPF 446_ SAGLR 451_ NCIGQ 456_ HFAII 461_ ECKVA 466_ VALTL 471_ LRFKL 476_ APDHS 481_ RPPQP 486_ VRQVV 491_ LKSKN 496_GIHVF
1: A cytochrome P450 monooxygenase that catalyzes the in-chain oxidation of fatty acids (PubMed:19090726, PubMed:29018033). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates lauric and myristic acids predominantly at the omega-4 and omega-2 positions, respectively (PubMed:19090726, PubMed:29018033). Catalyzes the epoxidation of double bonds of polyunsaturated fatty acids (PUFA). Displays an absolute stereoselectivity in the epoxidation of arachidonic acid producing the 14(S),15(R)-epoxyeicosatrienoic acid (EET) enantiomer (PubMed:29018033). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:19090726, PubMed:29018033)