RNA N6-adenosine-methyltransferase METTL16 (Methyltransferase 10 domain-containing protein) (Methyltransferase-like protein 16) (N6-adenosine-methyltransferase METTL16) (EC 2.1.1.348) (U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase) (EC 2.1.1.346)
1_MALSK 6_ SMHAR 11_ NRYKD 16_ KPPDF 21_ AYLAS 26_ KYPDF 31_ KQHVQ 36_ INLNG 41_ RVSLN 46_ FKDPE 51_ AVRAL 56_ TCTLL 61_ REDFG 66_ LSIDI 71_ PLERL 76_ IPTVP 81_ LRLNY 86_ IHWVE 91_ DLIGH 96_ QDSDK 101_ STLRR 106_ GIDIG 111_ TGASC 116_ IYPLL 121_ GATLN 126_ GWYFL 131_ ATEVD 136_ DMCFN 141_ YAKKN 146_ VEQNN 151_ LSDLI 156_ KVVKV 161_ PQKTL 166_ LMDAL 171_ KEESE 176_ IIYDF 181_ CMCNP 186_ PFFAN 191_ QLEAK 196_ GVNSR 201_ NPRRP 206_ PPSSV 211_ NTGGI 216_ TEIMA 221_ EGGEL 226_ EFVKR 231_ IIHDS 236_ LQLKK 241_ RLRWY 246_ SCMLG 251_ KKCSL 256_ APLKE 261_ ELRIQ 266_ GVPKV 271_ TYTEF 276_ CQGRT 281_ MRWAL 286_ AWSFY 291_ DDVTV 296_ PSPPS 301_ KRRKL 306_ EKPRK 311_ PITFV 316_ VLASV 321_ MKELS 326_ LKASP 331_ LRSET 336_ AEGIV 341_ VVTTW 346_ IEKIL 351_ TDLKV 356_ QHKRV 361_ PCGKE 366_ EVSLF 371_ LTAIE 376_ NSWIH 381_ LRRKK 386_ RERVR 391_ QLREV 396_ PRAPE 401_ DVIQA 406_ LEEKK 411_ PTPKE 416_ SGNSQ 421_ ELARG 426_ PQERT 431_ PCGPA 436_ LREGE 441_ AAAVE 446_ GPCPS 451_ QESLS 456_ QEENP 461_ EPTED 466_ ERSEE 471_ KGGVE 476_ VLESC 481_ QGSSN 486_ GAQDQ 491_ EASEQ 496_ FGSPV 501_ AERGK 506_ RLPGV 511_ AGQYL 516_ FKCLI 521_ NVKKE 526_ VDDAL 531_ VEMHW 536_ VEGQN 541_ RDLMN 546_ QLCTY 551_ IRNQI 556_FRLVA
1: RNA N6-methyltransferase that methylates adenosine residues at the N(6) position of a subset of RNAs and is involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts (PubMed:28525753, PubMed:30197297, PubMed:30197299, PubMed:33428944, PubMed:33930289). Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs) (PubMed:28525753). In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs: requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure (PubMed:28525753, PubMed:30197297, PubMed:30197299). Plays a key role in S-adenosyl-L-methionine homeostasis by mediating N6-methylation of MAT2A mRNAs, altering splicing of MAT2A transcripts: in presence of S-adenosyl-L-methionine, binds the 3'-UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, preventing recognition of their 3'-splice site by U2AF1/U2AF35, thereby inhibiting splicing and protein production of S-adenosylmethionine synthase (PubMed:28525753, PubMed:33930289). In S-adenosyl-L-methionine-limiting conditions, binds the 3'-UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, preventing N6-methylation and promoting expression of MAT2A (PubMed:28525753). In addition to mRNAs, also able to mediate N6-methylation of U6 small nuclear RNA (U6 snRNA): specifically N6-methylates adenine in position 43 of U6 snRNAs (PubMed:28525753, PubMed:29051200, PubMed:32266935). Also able to bind various lncRNAs, such as 7SK snRNA (7SK RNA) or 7SL RNA (PubMed:29051200). Specifically binds the 3'-end of the MALAT1 long non-coding RNA (PubMed:27872311)