Serine/threonine-protein kinase VRK2 (EC 2.7.11.1) (Vaccinia-related kinase 2)
1_MPPKR 6_ NEKYK 11_ LPIPF 16_ PEGKV 21_ LDDME 26_ GNQWV 31_ LGKKI 36_ GSGGF 41_ GLIYL 46_ AFPTN 51_ KPEKD 56_ ARHVV 61_ KVEYQ 66_ ENGPL 71_ FSELK 76_ FYQRV 81_ AKKDC 86_ IKKWI 91_ ERKQL 96_ DYLGI 101_ PLFYG 106_ SGLTE 111_ FKGRS 116_ YRFMV 121_ MERLG 126_ IDLQK 131_ ISGQN 136_ GTFKK 141_ STVLQ 146_ LGIRM 151_ LDVLE 156_ YIHEN 161_ EYVHG 166_ DIKAA 171_ NLLLG 176_ YKNPD 181_ QVYLA 186_ DYGLS 191_ YRYCP 196_ NGNHK 201_ QYQEN 206_ PRKGH 211_ NGTIE 216_ FTSLD 221_ AHKGV 226_ ALSRR 231_ SDVEI 236_ LGYCM 241_ LRWLC 246_ GKLPW 251_ EQNLK 256_ DPVAV 261_ QTAKT 266_ NLLDE 271_ LPQSV 276_ LKWAP 281_ SGSSC 286_ CEIAQ 291_ FLVCA 296_ HSLAY 301_ DEKPN 306_ YQALK 311_ KILNP 316_ HGIPL 321_ GPLDF 326_ STKGQ 331_ SINVH 336_ TPNSQ 341_ KVDSQ 346_ KAATK 351_ QVNKA 356_ HNRLI 361_ EKKVH 366_ SERSA 371_ ESCAT 376_ WKVQK 381_ EEKLI 386_ GLMNN 391_ EAAQE 396_ STRRR 401_ QKYQE 406_ SQEPL 411_ NEVNS 416_ FPQKI 421_ SYTQF 426_ PNSFY 431_ EPHQD 436_ FTSPD 441_ IFKKS 446_ RSPSW 451_ YKYTS 456_ TVSTG 461_ ITDLE 466_ SSTGL 471_ WPTIS 476_ QFTLS 481_ EETNA 486_ DVYYY 491_ RIIIP 496_ VLLML 501_VFLAL
1: Serine/threonine kinase that regulates several signal transduction pathways (PubMed:14645249, PubMed:16495336, PubMed:16704422, PubMed:17709393, PubMed:18286207, PubMed:18617507, PubMed:20679487). Isoform 1 modulates the stress response to hypoxia and cytokines, such as interleukin-1 beta (IL1B) and this is dependent on its interaction with MAPK8IP1, which assembles mitogen-activated protein kinase (MAPK) complexes (PubMed:17709393). Inhibition of signal transmission mediated by the assembly of MAPK8IP1-MAPK complexes reduces JNK phosphorylation and JUN-dependent transcription (PubMed:18286207). Phosphorylates 'Thr-18' of p53/TP53, histone H3, and may also phosphorylate MAPK8IP1 (PubMed:16704422). Phosphorylates BANF1 and disrupts its ability to bind DNA and reduces its binding to LEM domain-containing proteins (PubMed:16495336). Down-regulates the transactivation of transcription induced by ERBB2, HRAS, BRAF, and MEK1 (PubMed:20679487). Blocks the phosphorylation of ERK in response to ERBB2 and HRAS (PubMed:20679487). Can also phosphorylate the following substrates that are commonly used to establish in vitro kinase activity: casein, MBP and histone H2B, but it is not sure that this is physiologically relevant (PubMed:14645249)
2: Phosphorylates 'Thr-18' of p53/TP53, as well as histone H3. Reduces p53/TP53 ubiquitination by MDM2, promotes p53/TP53 acetylation by EP300 and thereby increases p53/TP53 stability and activity