3'-5' exoribonuclease 1 (EC 3.1.-.-) (3'-5' exonuclease ERI1) (Eri-1 homolog) (Histone mRNA 3'-end-specific exoribonuclease) (Histone mRNA 3'-exonuclease 1) (Protein 3'hExo) (HEXO)
1_MEDPQ 6_ SKEPA 11_ GEAVA 16_ LALLE 21_ SPRPE 26_ GGEEP 31_ PRPSP 36_ EETQQ 41_ CKFDG 46_ QETKG 51_ SKFIT 56_ SSASD 61_ FSDPV 66_ YKEIA 71_ ITNGC 76_ INRMS 81_ KEELR 86_ AKLSE 91_ FKLET 96_ RGVKD 101_ VLKKR 106_ LKNYY 111_ KKQKL 116_ MLKES 121_ NFADS 126_ YYDYI 131_ CIIDF 136_ EATCE 141_ EGNPP 146_ EFVHE 151_ IIEFP 156_ VVLLN 161_ THTLE 166_ IEDTF 171_ QQYVR 176_ PEINT 181_ QLSDF 186_ CISLT 191_ GITQD 196_ QVDRA 201_ DTFPQ 206_ VLKKV 211_ IDWMK 216_ LKELG 221_ TKYKY 226_ SLLTD 231_ GSWDM 236_ SKFLN 241_ IQCQL 246_ SRLKY 251_ PPFAK 256_ KWINI 261_ RKSYG 266_ NFYKV 271_ PRSQT 276_ KLTIM 281_ LEKLG 286_ MDYDG 291_ RPHCG 296_ LDDSK 301_ NIARI 306_ AVRML 311_ QDGCE 316_ LRINE 321_ KMHAG 326_ QLMSV 331_ SSSLP 336_ IEGTP 341_PPQMP
1: RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication (PubMed:14536070, PubMed:16912046, PubMed:17135487, PubMed:37352860). A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient 3'-end histone mRNA exonuclease activity and degradation of RNA substrates (PubMed:14536070, PubMed:16912046, PubMed:17135487). Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi) (PubMed:14961122). Required for binding the 5'-ACCCA-3' sequence present in stem-loop structure (PubMed:14536070, PubMed:16912046). Able to bind other mRNAs (PubMed:14536070, PubMed:16912046). Required for 5.8S rRNA 3'-end processing (PubMed:37352860). Also binds to 5.8s ribosomal RNA (By similarity). Binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs (PubMed:14536070, PubMed:16912046, PubMed:17135487). In vitro, does not have sequence specificity (PubMed:17135487). In vitro, has weak DNA exonuclease activity (PubMed:17135487). In vitro, shows biphasic kinetics such that there is rapid hydrolysis of the last three unpaired RNA nucleotides in the 39 flanking sequence followed by a much slower cleavage through the stem that occurs over a longer incubation period in the order of hours (PubMed:17135487). ERI1-mediated RNA metabolism plays a key role in chondrogenesis (PubMed:37352860)