Tetratricopeptide repeat protein 5 (TPR repeat protein 5) (Stress-responsive activator of p300) (Protein Strap)
1_MMADE 6_ EEEVK 11_ PILQK 16_ LQELV 21_ DQLYS 26_ FRDCY 31_ FETHS 36_ VEDAG 41_ RKQQD 46_ VQKEM 51_ EKTLQ 56_ QMEEV 61_ VGSVQ 66_ GKAQV 71_ LMLTG 76_ KALNV 81_ TPDYS 86_ PKAEE 91_ LLSKA 96_ VKLEP 101_ ELVEA 106_ WNQLG 111_ EVYWK 116_ KGDVA 121_ AAHTC 126_ FSGAL 131_ THCRN 136_ KVSLQ 141_ NLSMV 146_ LRQLR 151_ TDTED 156_ EHSHH 161_ VMDSV 166_ RQAKL 171_ AVQMD 176_ VHDGR 181_ SWYIL 186_ GNSYL 191_ SLYFS 196_ TGQNP 201_ KISQQ 206_ ALSAY 211_ AQAEK 216_ VDRKA 221_ SSNPD 226_ LHLNR 231_ ATLHK 236_ YEESY 241_ GEALE 246_ GFSRA 251_ AALDP 256_ AWPEP 261_ RQREQ 266_ QLLEF 271_ LDRLT 276_ SLLES 281_ KGKVK 286_ TKKLQ 291_ SMLGS 296_ LRPAH 301_ LGPCS 306_ DGHYQ 311_ SASGQ 316_ KVTLE 321_ LKPLS 326_ TLQPG 331_ VNSGA 336_ VILGK 341_ VVFSL 346_ TTEEK 351_ VPFTF 356_ GLVDS 361_ DGPCY 366_ AVMVY 371_ NIVQS 376_ WGVLI 381_ GDSVA 386_ IPEPN 391_ LRLHR 396_ IQHKG 401_ KDYSF 406_ SSVRV 411_ ETPLL 416_ LVVNG 421_ KPQGS 426_ SSQAV 431_ATVAS
1: Cofactor involved in the regulation of various cellular mechanisms such as actin regulation, autophagy, chromatin regulation and DNA repair (PubMed:18451878, PubMed:31727855). In non-stress conditions, interacts with cofactor JMY in the cytoplasm which prevents JMY's actin nucleation activity and ability to activate the Arp2/3 complex. Acts as a negative regulator of nutrient stress-induced autophagy by preventing JMY's interaction with MAP1LC3B, thereby preventing autophagosome formation (By similarity). Involves in tubulin autoregulation by promoting its degradation in response to excess soluble tubulin (PubMed:31727855). To do so, associates with the active ribosome near the ribosome exit tunnel and with nascent tubulin polypeptides early during their translation, triggering tubulin mRNA-targeted degradation (PubMed:31727855). Following DNA damage, phosphorylated by DNA damage responsive protein kinases ATM and CHEK2, leading to its nuclear accumulation and stability. Nuclear TTC5/STRAP promotes the assembly of a stress-responsive p53/TP53 coactivator complex, which includes the coactivators JMY and p300, thereby increasing p53/TP53-dependent transcription and apoptosis. Also recruits arginine methyltransferase PRMT5 to p53/TP53 when DNA is damaged, allowing PRMT5 to methylate p53/TP53. In DNA stress conditions, also prevents p53/TP53 degradation by E3 ubiquitin ligase MDM2 (By similarity). Upon heat-shock stress, forms a chromatin-associated complex with heat-shock factor 1 HSF1 and p300/EP300 to stimulate heat-shock-responsive transcription, thereby increasing cell survival (PubMed:18451878). Mitochondrial TTC5/STRAP interacts with ATP synthase subunit beta ATP5F1B which decreased ATP synthase activity and lowers mitochondrial ATP production, thereby regulating cellular respiration and mitochondrial-dependent apoptosis. Mitochondrial TTC5/STRAP also regulates p53/TP53-mediated apoptosis (By similarity)