E3 SUMO-protein ligase PIAS4 (EC 2.3.2.27) (PIASy) (Protein inhibitor of activated STAT protein 4) (Protein inhibitor of activated STAT protein gamma) (PIAS-gamma)
1_MAAEL 6_ VEAKN 11_ MVMSF 16_ RVSDL 21_ QMLLG 26_ FVGRS 31_ KSGLK 36_ HELVT 41_ RALQL 46_ VQFDC 51_ SPELF 56_ KKIKE 61_ LYETR 66_ YAKKN 71_ SEPAP 76_ QPHRP 81_ LDPLT 86_ MHSTY 91_ DRAGA 96_ VPRTP 101_ LAGPN 106_ IDYPV 111_ LYGKY 116_ LNGLG 121_ RLPAK 126_ TLKPE 131_ VRLVK 136_ LPFFN 141_ MLDEL 146_ LKPTE 151_ LVPQN 156_ NEKLQ 161_ ESPCI 166_ FALTP 171_ RQVEL 176_ IRNSR 181_ ELQPG 186_ VKAVQ 191_ VVLRI 196_ CYSDT 201_ SCPQE 206_ DQYPP 211_ NIAVK 216_ VNHSY 221_ CSVPG 226_ YYPSN 231_ KPGVE 236_ PKRPC 241_ RPINL 246_ THLMY 251_ LSSAT 256_ NRITV 261_ TWGNY 266_ GKSYS 271_ VALYL 276_ VRQLT 281_ SSELL 286_ QRLKT 291_ IGVKH 296_ PELCK 301_ ALVKE 306_ KLRLD 311_ PDSEI 316_ ATTGV 321_ RVSLI 326_ CPLVK 331_ MRLSV 336_ PCRAE 341_ TCAHL 346_ QCFDA 351_ VFYLQ 356_ MNEKK 361_ PTWMC 366_ PVCDK 371_ PAPYD 376_ QLIID 381_ GLLSK 386_ ILSEC 391_ EDADE 396_ IEYLV 401_ DGSWC 406_ PIRAE 411_ KERSC 416_ SPQGA 421_ ILVLG 426_ PSDAN 431_ GLLPA 436_ PSVNG 441_ SGALG 446_ STGGG 451_ GPVGS 456_ MENGK 461_ PGADV 466_ VDLTL 471_ DSSSS 476_ SEDEE 481_ EEEEE 486_ EEDED 491_ EEGPR 496_ PKRRC 501_PFQKG
1: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor (PubMed:12511558, PubMed:12631292, PubMed:12727872, PubMed:15831457, PubMed:15976810, PubMed:22508508, PubMed:32832608). Mediates sumoylation of ALKBH5, AXIN1, CEBPA, KLF8, GATA2, PARK7, HERC2, MYB, TCF4 and RNF168 (PubMed:12223491, PubMed:12511558, PubMed:12631292, PubMed:12727872, PubMed:12750312, PubMed:15831457, PubMed:15976810, PubMed:16617055, PubMed:22508508, PubMed:34048572). Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway (PubMed:11388671). Involved in gene silencing (PubMed:11248056). In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations (PubMed:12727872, PubMed:15831457). Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed:21965678). Binds to AT-rich DNA sequences, known as matrix or scaffold attachment regions (MARs/SARs) (By similarity). Catalyzes conjugation of SUMO2 to KAT5 in response to DNA damage, facilitating repair of DNA double-strand breaks (DSBs) via homologous recombination (HR) (PubMed:32832608). Mediates sumoylation of PARP1 in response to PARP1 trapping to chromatin (PubMed:35013556). Mediates sumoylation of KLF8, repressiing KLF8 transcriptional activity and cell cycle progression into G(1) phase (PubMed:16617055). Sumoylates ALKBH5 downstream of MAPK8/JNK1 and MAPK9/JNK2 in response to reactive oxygen species (ROS), inhibiting ALKBH5 RNA demethylase activity (PubMed:34048572)