RNA polymerase II-associated factor 1 homolog (hPAF1) (Pancreatic differentiation protein 2)
1_MAPTI 6_ QTQAQ 11_ REDGH 16_ RPNSH 21_ RTLPE 26_ RSGVV 31_ CRVKY 36_ CNSLP 41_ DIPFD 46_ PKFIT 51_ YPFDQ 56_ NRFVQ 61_ YKATS 66_ LEKQH 71_ KHDLL 76_ TEPDL 81_ GVTID 86_ LINPD 91_ TYRID 96_ PNVLL 101_ DPADE 106_ KLLEE 111_ EIQAP 116_ TSSKR 121_ SQQHA 126_ KVVPW 131_ MRKTE 136_ YISTE 141_ FNRYG 146_ ISNEK 151_ PEVKI 156_ GVSVK 161_ QQFTE 166_ EEIYK 171_ DRDSQ 176_ ITAIE 181_ KTFED 186_ AQKSI 191_ SQHYS 196_ KPRVT 201_ PVEVM 206_ PVFPD 211_ FKMWI 216_ NPCAQ 221_ VIFDS 226_ DPAPK 231_ DTSGA 236_ AALEM 241_ MSQAM 246_ IRGMM 251_ DEEGN 256_ QFVAY 261_ FLPVE 266_ ETLKK 271_ RKRDQ 276_ EEEMD 281_ YAPDD 286_ VYDYK 291_ IAREY 296_ NWNVK 301_ NKASK 306_ GYEEN 311_ YFFIF 316_ REGDG 321_ VYYNE 326_ LETRV 331_ RLSKR 336_ RAKAG 341_ VQSGT 346_ NALLV 351_ VKHRD 356_ MNEKE 361_ LEAQE 366_ ARKAQ 371_ LENHE 376_ PEEEE 381_ EEEME 386_ TEEKE 391_ AGGSD 396_ EEQEK 401_ GSSSE 406_ KEGSE 411_ DEHSG 416_ SESER 421_ EEGDR 426_ DEASD 431_ KSGSG 436_ EDESS 441_ EDEAR 446_ AARDK 451_ EEIFG 456_ SDADS 461_ EDDAD 466_ SDDED 471_ RGQAQ 476_ GGSDN 481_ DSDSG 486_ SNGGG 491_ QRSRS 496_ HSRSA 501_ SPFPS 506_ GSEHS 511_ AQEDG 516_ SEAAA 521_ SDSSE 526_ADSDS
1: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both independently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Connects PAF1C with the RNF20/40 E3 ubiquitin-protein ligase complex. Involved in polyadenylation of mRNA precursors. Has oncogenic activity in vivo and in vitro