Uncharacterized protein C12orf29
1_MKRLG 6_ SVQRK 11_ MPCVF 16_ VTEVK 21_ EEPSS 26_ KREHQ 31_ PFKVL 36_ ATETV 41_ SHKAL 46_ DADIY 51_ SAIPT 56_ EKVDG 61_ TCCYV 66_ TTYKD 71_ QPYLW 76_ ARLDR 81_ KPNKQ 86_ AEKRF 91_ KNFLH 96_ SKENP 101_ KEFFW 106_ NVEED 111_ FKPAP 116_ ECWIP 121_ AKETE 126_ QINGN 131_ PVPDE 136_ NGHIP 141_ GWVPV 146_ EKNNK 151_ QYCWH 156_ SSVVN 161_ YEFEI 166_ ALVLK 171_ HHPDD 176_ SGLLE 181_ ISAVP 186_ LSDLL 191_ EQTLE 196_ LIGTN 201_ INGNP 206_ YGLGS 211_ KKHPL 216_ HLLIP 221_ HGAFQ 226_ IRNLP 231_ SLKHN 236_ DLVSW 241_ FEDCK 246_ EGKIE 251_ GIVWH 256_ CSDGC 261_ LIKVH 266_ RHHLG 271_ LCWPI 276_ PDTYM 281_ NSRPV 286_ IINMN 291_ LNKCD 296_ SAFDI 301_ KCLFN 306_ HFLKI 311_ DNQKF 316_VRLKD
1: Functions as an RNA ligase, in vitro (PubMed:36792600). The ligation reaction entails three nucleotidyl transfer steps (PubMed:36792600). In the first step, the RNA ligase reacts with ATP in the absence of nucleic acid to form a covalent ligase-AMP intermediate and release pyrophosphate (PubMed:36792600). In step 2, the ligase-AMP binds to the nucleic acid and transfers the adenylate to the 5'-PO4 terminus to form an adenylylated intermediate (PubMed:36792600). In step 3, the RNA ligase directs the attack of the 3'-OH on the 5'-phosphoanhydride linkage, resulting in a repaired 3'-5' phosphodiester and release of AMP (PubMed:36792600). Exhibits selectivity for single-stranded RNA substrates and may not have nick-sealing activity on double-stranded DNA-RNA hybrids (PubMed:36792600). May play a role in maintaining RNA integrity under stress conditions, for example in response to reactive oxygen species (ROS) (PubMed:36792600)