tRNA-uridine aminocarboxypropyltransferase 2 (EC 2.5.1.25) (DTW domain-containing protein 2)
1_MESQK 6_ EARTL 11_ QEPVA 16_ RPSGA 21_ SSSQT 26_ PNDKE 31_ RREGG 36_ AVPAA 41_ AALGA 46_ EADDD 51_ SADGL 56_ WELPV 61_ EPAER 66_ RPECT 71_ RCSRP 76_ QKVCL 81_ CPFLP 86_ AHPLH 91_ ISTHL 96_ YIIQH 101_ PAEEN 106_ KVLRT 111_ VPLLA 116_ ACLPQ 121_ DKCKV 126_ KIGRR 131_ FSEER 136_ DPELS 141_ TVCRK 146_ SGTLI 151_ LYPGA 156_ EAANL 161_ EEFIL 166_ DSPVY 171_ PSTII 176_ IIDGT 181_ WSQAK 186_ DIFYK 191_ NSLFR 196_ HPKQV 201_ QLKTS 206_ ISSQY 211_ VIRMQ 216_ PTNRC 221_ LSTLE 226_ CAAVA 231_ LSILE 236_ KNNYI 241_ QETLL 246_ RPLQA 251_ LCSFQ 256_ LQHGA 261_ QIRLS 266_ KEHLL 271_ KNGLY 276_ PKPMP 281_ KNKRK 286_ LRKME 291_LLMNS
1: Catalyzes the formation of 3-(3-amino-3-carboxypropyl)uridine (acp3U) at position 20a in the D-loop of several cytoplasmic tRNAs (acp3U(20a)) (PubMed:31804502, PubMed:39173631). Also has a weak activity to form acp3U at position 20 in the D-loop of tRNAs (acp3U(20)) (PubMed:31804502). Involved in glycoRNA biosynthesis by mediating formation of acp3U, which acts as an attachment site for N-glycans on tRNAs (PubMed:39173631). GlycoRNAs consist of RNAs modified with secretory N-glycans that are presented on the cell surface (PubMed:39173631)