tRNA-splicing endonuclease subunit Sen2 (EC 4.6.1.16) (tRNA-intron endonuclease Sen2) (HsSen2)
1_MAEAV 6_ FHAPK 11_ RKRRV 16_ YETYE 21_ SPLPI 26_ PFGQD 31_ HGPLK 36_ EFKIF 41_ RAEMI 46_ NNNVI 51_ VRNAE 56_ DIEQL 61_ YGKGY 66_ FGKGI 71_ LSRSR 76_ PSFTI 81_ SDPKL 86_ VAKWK 91_ DMKTN 96_ MPIIT 101_ SKRYQ 106_ HSVEW 111_ AAELM 116_ RRQGQ 121_ DESTV 126_ RRILK 131_ DYTKP 136_ LEHPP 141_ VKRNE 146_ EAQVH 151_ DKLNS 156_ GMVSN 161_ MEGTA 166_ GGERP 171_ SVVNG 176_ DSGKS 181_ GGVGD 186_ PREPL 191_ GCLQE 196_ GSGCH 201_ PTTES 206_ FEKSV 211_ REDAS 216_ PLPHV 221_ CCCKQ 226_ DALIL 231_ QRGLH 236_ HEDGS 241_ QHIGL 246_ LHPGD 251_ RGPDH 256_ EYVLV 261_ EEAEC 266_ AMSER 271_ EAAPN 276_ EELVQ 281_ RNRLI 286_ CRRNP 291_ YRIFE 296_ YLQLS 301_ LEEAF 306_ FLVYA 311_ LGCLS 316_ IYYEK 321_ EPLTI 326_ VKLWK 331_ AFTVV 336_ QPTFR 341_ TTYMA 346_ YHYFR 351_ SKGWV 356_ PKVGL 361_ KYGTD 366_ LLLYR 371_ KGPPF 376_ YHASY 381_ SVIIE 386_ LVDDH 391_ FEGSL 396_ RRPLS 401_ WKSLA 406_ ALSRV 411_ SVNVS 416_ KELML 421_ CYLIK 426_ PSTMT 431_ DKEME 436_ SPECM 441_ KRIKV 446_ QEVIL 451_ SRWVS 456_SRERS
1: Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Isoform 1 probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. Isoform 2 is responsible for processing a yet unknown RNA substrate. The complex containing isoform 2 is not able to cleave pre-tRNAs properly, although it retains endonucleolytic activity