Carbohydrate sulfotransferase 4 (EC 2.8.2.-) (Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3) (GST-3) (High endothelial cells N-acetylglucosamine 6-O-sulfotransferase) (HEC-GlcNAc6ST) (L-selectin ligand sulfotransferase) (LSST) (N-acetylglucosamine 6-O-sulfotransferase 2) (GlcNAc6ST-2) (Gn6st-2)
1_MLLPK 6_ KMKLL 11_ LFLVS 16_ QMAIL 21_ ALFFH 26_ MYSHN 31_ ISSLS 36_ MKAQP 41_ ERMHV 46_ LVLSS 51_ WRSGS 56_ SFVGQ 61_ LFGQH 66_ PDVFY 71_ LMEPA 76_ WHVWM 81_ TFKQS 86_ TAWML 91_ HMAVR 96_ DLIRA 101_ VFLCD 106_ MSVFD 111_ AYMEP 116_ GPRRQ 121_ SSLFQ 126_ WENSR 131_ ALCSA 136_ PACDI 141_ IPQDE 146_ IIPRA 151_ HCRLL 156_ CSQQP 161_ FEVVE 166_ KACRS 171_ YSHVV 176_ LKEVR 181_ FFNLQ 186_ SLYPL 191_ LKDPS 196_ LNLHI 201_ VHLVR 206_ DPRAV 211_ FRSRE 216_ RTKGD 221_ LMIDS 226_ RIVMG 231_ QHEQK 236_ LKKED 241_ QPYYV 246_ MQVIC 251_ QSQLE 256_ IYKTI 261_ QSLPK 266_ ALQER 271_ YLLVR 276_ YEDLA 281_ RAPVA 286_ QTSRM 291_ YEFVG 296_ LEFLP 301_ HLQTW 306_ VHNIT 311_ RGKGM 316_ GDHAF 321_ HTNAR 326_ DALNV 331_ SQAWR 336_ WSLPY 341_ EKVSR 346_ LQKAC 351_ GDAMN 356_ LLGYR 361_ HVRSE 366_ QEQRN 371_ LLLDL 376_ LSTWT 381_VPEQI
1: Sulfotransferase involved in SELL/L-selectin ligand biosynthesis pathway. Catalyzes the transfer of the sulfate group from 3'-phospho-5'-adenylyl sulfate (PAPS) onto the hydroxyl group at C-6 position of the non-reducing N-acetylglucosamine (GlcNAc) residue within O-linked mucin-type glycans. Contributes to generate sialyl 6-sulfo Lewis X determinant (also known as MECA-79 epitope) for SELL recognition, a prerequisite for continuous lymphocyte homing into peripheral lymph nodes and antigen immune surveillance (PubMed:10330415, PubMed:11439191, PubMed:11726653, PubMed:12107080). Transfers the sulfate group primarily on core 2 GlcNAcbeta1-6(Galbeta1-3)GalNAcalphaSer/Thr and extended core 1 GlcNAcbeta1-3Galbeta1-3GalNAcalphaSer/Thr based O-linked glycans on CD34 and GLYCAM1 peripheral node addressins (PNAds) expressed on the lumenal side of high endothelial venules (HEVs) (PubMed:11439191). The recognition of PNAds by SELL initiates a multistep process comprising tethering and rolling of blood lymphocytes on HEVs against the blood flow, followed by chemokine signaling, integrin-mediated lymphocyte adhesion onto endothelial cells and lymphocyte transendothelial migration. Modulates rolling velocity and differential T and B lymphocyte recruitment into peripheral lymph nodes, with a major role in B lymphocyte homing. Might be redundant in sulfation of MADCAM1 and lymphocyte trafficking to mesenteric lymph nodes (By similarity). Can also sulfonate core 3 GlcNAcbeta1-3GalNAc-R based glycans as well as GlcNAcbeta1-3Galbeta1-Glc, GlcNAcbeta1-6ManOMe and GlcNAcbeta1-2Man oligosaccharides, which might be ectopically expressed during tumorigenesis (PubMed:11439191, PubMed:11726653, PubMed:12107080)