Thiosulfate:glutathione sulfurtransferase (TST) (EC 2.8.1.-)
1_MAGAP 6_ TVSLP 11_ ELRSL 16_ LASGR 21_ ARLFD 26_ VRSRE 31_ EAAAG 36_ TIPGA 41_ LNIPV 46_ SELES 51_ ALQME 56_ PAAFQ 61_ ALYSA 66_ EKPKL 71_ EDEHL 76_ VFFCQ 81_ MGKRG 86_ LQATQ 91_ LARSL 96_ GYTGA 101_ RNYAG 106_AYREW
1: Thiosulfate:glutathione sulfurtransferase (TST) required to produce S-sulfanylglutathione (GSS(-)), a central intermediate in hydrogen sulfide metabolism (PubMed:24981631). Provides the link between the first step in mammalian H(2)S metabolism performed by the sulfide:quinone oxidoreductase (SQOR) which catalyzes the conversion of H(2)S to thiosulfate, and the sulfur dioxygenase (SDO) which uses GSS(-) as substrate (PubMed:24981631). The thermodynamic coupling of the irreversible SDO and reversible TST reactions provides a model for the physiologically relevant reaction with thiosulfate as the sulfane donor (PubMed:24981631). GSS(-) spontaneously reacts with glutathione to form glutathione disulfide (Probable)